National Center for Natural Products Research, University of Mississippi, University, MS 38677, USA.
J Chromatogr A. 2011 May 20;1218(20):2995-3001. doi: 10.1016/j.chroma.2011.03.037. Epub 2011 Apr 7.
The experimental technique of mass spectrometric tracer pulse chromatography was used to determine the void volume, i.e., the total volume of eluent in the column, and the volume of eluent moving freely through the column, i.e., mobile phase volume, for a series of eluents with a C(18)-bonded RPLC column. The interstitial volume of the column was determined by size exclusion chromatography. In order to evaluate the utility of the accessible volumes determined from the retention volumes of homologous solutes, the accessible volume of the column was determined as a function of eluent composition and temperature with polystyrene and polyethylene glycol samples using Martin's Rule. Comparison of these four measured volumes indicated that the experimentally measured accessible volumes did not correspond to either the void volumes, mobile phase volumes or interstitial volumes.
采用质量色谱示踪脉冲实验技术，确定了一系列 C(18)键合反相高效液相色谱柱洗脱液的空隙体积（即柱内洗脱液的总体积）和自由通过柱的洗脱液体积（即流动相体积）。通过尺寸排阻色谱法测定柱的内体积。为了评估从同系物保留体积确定的可及体积的实用性，使用马丁规则，以聚苯乙烯和聚乙二醇样品为对象，研究了柱的可及体积随洗脱液组成和温度的变化关系。对这四个测量体积的比较表明，实验测量的可及体积与空隙体积、流动相体积或内体积均不相符。
