Division of Animal Biochemistry, National Dairy Research Institute, Karnal-132001, Haryana, India.
Res Vet Sci. 2012 Apr;92(2):207-12. doi: 10.1016/j.rvsc.2011.03.018. Epub 2011 Apr 8.
The present study was designed to see the effects of Angiotensin-II (Ang-II) on buffalo sperm capacitation, acrosome reaction (AR), and its relation to nitric oxide (NO()) production. The extent of capacitation or AR was determined by dual staining while the NO() production was determined by spectrophotometry. The results thus obtained revealed that Ang-II induced capacitation in a concentration and time dependent manner and 200 nM Ang-II was found to be optimal for capacitation as it was comparable to heparin treatment (50.7±2.45% vs. 51.66±2.33%). In capacitated cells the extent of AR induced by Ang-II was significantly higher than the untreated control (48.13±2.31% vs. 22.16±2.11%) and comparable to lysophosphatidyl Choline (LPC) treatment (51.56±1.94%). The NO() production during Ang-II induced capacitation and AR was gradual and time dependent. These levels were significantly higher when compared to control (3.65±0.53 nmoles/10(8)cells vs. 9.12±0.30 nmoles/10(8)cells). All the actions of Ang-II were inhibited in the presence of Losartan but not PD123319, indicating the role of AT1 receptors in these actions. Further the NO() production was also significantly inhibited in the presence of neomycin and trifluoperazine pointing towards the role of phosphoinositide pathway in this process. In conclusion, Ang-II has a concentration and time dependent effect on buffalo sperm capacitation and AR, mediated via the AT1 receptors. Its effect on NO() production may be indirect involving the phosphoinositide pathway.
本研究旨在观察血管紧张素 II（Ang-II）对水牛精子获能、顶体反应（AR）的影响及其与一氧化氮（NO()）产生的关系。通过双重染色来确定获能或 AR 的程度，而通过分光光度法来确定 NO()的产生。结果表明，Ang-II 以浓度和时间依赖的方式诱导获能，200 nM Ang-II 被发现是最佳的获能浓度，因为它与肝素处理（50.7±2.45% vs. 51.66±2.33%）相当。在获能细胞中，Ang-II 诱导的 AR 程度明显高于未处理的对照组（48.13±2.31% vs. 22.16±2.11%），与溶血磷脂酰胆碱（LPC）处理相当（51.56±1.94%）。在 Ang-II 诱导的获能和 AR 过程中，NO()的产生是逐渐的，且依赖于时间。与对照组相比，这些水平明显升高（3.65±0.53 nmoles/10(8)cells vs. 9.12±0.30 nmoles/10(8)cells）。在 Losartan 存在的情况下，Ang-II 的所有作用都被抑制，但 PD123319 没有，表明 AT1 受体在这些作用中起作用。此外，在新霉素和三氟拉嗪存在的情况下，NO()的产生也明显受到抑制，这表明在这个过程中磷酸肌醇途径起作用。总之，Ang-II 对水牛精子的获能和 AR 有浓度和时间依赖性的影响，通过 AT1 受体介导。其对 NO()产生的影响可能是间接的，涉及磷酸肌醇途径。
