Unis A S, Vincent J G, Dillon B
Department of Psychiatry and Behavioral Sciences, University of Washington, Seattle 98195.
Life Sci. 1990;47(25):PL151-5. doi: 10.1016/0024-3205(90)90279-z.
Using the technique of in vitro receptor autoradiography to slide-mounted tissue sections, we studied the suitability of [3H]-YM-09151-2 as a ligand for labeling D-2 receptors in adult F344 rat brains. Specific [3H]-YM-09151-2 binding accounted for 70-80% of the total bound ligand and reached equilibrium after a 60-90 minute incubation. Scatchard analysis revealed a Kd of 626 pM. The apparent Bmax was 23.2 fmol/tissue section. Autoradiographs demonstrated high grain densities in the striatum and olfactory tubercle. Diffuse specific binding was also observed in the cortex.
利用体外受体放射自显影技术对载玻片上的组织切片进行研究，我们考察了[3H]-YM-09151-2作为标记成年F344大鼠脑中D-2受体配体的适用性。特异性[3H]-YM-09151-2结合占总结合配体的70-80%，孵育60-90分钟后达到平衡。Scatchard分析显示解离常数（Kd）为626皮摩尔。表观最大结合容量（Bmax）为23.2飞摩尔/组织切片。放射自显影片显示纹状体和嗅结节中有高颗粒密度。在皮层中也观察到弥漫性特异性结合。
