School of Veterinary Sciences, University of Queensland, Gatton, QLD 4343, Australia.
Vet Parasitol. 2011 Aug 25;180(3-4):358-62. doi: 10.1016/j.vetpar.2011.03.021. Epub 2011 Mar 21.
Trypanosoma vivax is major cause of animal trypanosomiasis and responsible for enormous economic burden in Africa and South America animal industry. T. vivax infections mostly run low parasitaemia with no apparent clinical symptoms, making diagnosis a challenge. This work reports the design and evaluation of a loop-mediated isothermal amplification (LAMP) test for detecting T. vivax DNA based on the nuclear satellite repeat sequence. The assay is rapid with results obtained within 35 min. The analytical sensitivity is ∼ 1 trypanosome/ml while that of the classical PCR tests ranged from 10 to 10(3)trypanosomes/ml. The T. vivax LAMP test reported here is simple, robust and has future potential in diagnosis of animal trypanosomiasis in the field.
布氏锥虫是动物锥虫病的主要原因，给非洲和南美洲的动物产业造成了巨大的经济负担。T. vivax 感染通常伴有低寄生虫血症，没有明显的临床症状，这使得诊断具有挑战性。本工作报道了一种基于核卫星重复序列的环介导等温扩增（LAMP）检测 T. vivax DNA 的设计和评估。该检测方法快速，结果可在 35 分钟内获得。分析灵敏度约为 1 个锥虫/ml，而经典 PCR 检测方法的灵敏度范围为 10 到 10(3)个锥虫/ml。这里报道的 T. vivax LAMP 检测方法简单、稳健，具有在现场诊断动物锥虫病的未来潜力。
