在无寄生虫血症期间使用聚合酶链反应（PCR）和环介导等温扩增技术（LAMP）检测活泼锥虫。
Detection of Trypanosoma vivax using PCR and LAMP during aparasitemic periods.
作者信息
Cadioli Fabiano Antonio, Fidelis Junior Otavio Luiz, Sampaio Paulo Henrique, dos Santos Giuliana Nascimento, André Marcos Rogério, Castilho Kayo José Garcia de Almeida, Machado Rosangela Zacarias
机构信息
FMVA, UNESP/Araçatuba, Rua Clóvis Pestana 793, 16050-470 Araçatuba, SP, Brazil.
FCAV, UNESP/Jaboticabal, Via de Acesso Paulo Donato Castellane s/n, 14884-900 Jaboticabal, SP, Brazil.
出版信息
Vet Parasitol. 2015 Nov 30;214(1-2):174-7. doi: 10.1016/j.vetpar.2015.09.001. Epub 2015 Sep 3.
Trypanosoma vivax affects cattle herds in Africa and Americas and has been spreading rapidly in Brazil, through introduction of animals with subclinical infections and without apparent parasitemia, which makes its diagnosis challenging. PCR and LAMP are effective in detecting the presence of T. vivax DNA in situations of low parasitemia. LAMP is simpler and faster technique than PCR, and can be performed in the field, with limited resources. In this study, the capacities of conventional PCR and LAMP for detecting T. vivax in bovine blood samples classified as aparasitemic were evaluated. The capacity of conventional PCR (56.25%) for detecting positive samples was lower than that of LAMP (93.73%). This may influence the choice of screening tests for cattle herds infected with T. vivax.
间日锥虫感染非洲和美洲的牛群，并且通过引入亚临床感染且无明显寄生虫血症的动物，这种寄生虫在巴西迅速传播，这使得其诊断具有挑战性。聚合酶链反应（PCR）和环介导等温扩增技术（LAMP）在低寄生虫血症情况下能有效检测间日锥虫DNA的存在。LAMP是一种比PCR更简单、更快速的技术，并且可以在资源有限的现场进行。在本研究中，评估了传统PCR和LAMP检测分类为无寄生虫血症的牛血样中间日锥虫的能力。传统PCR检测阳性样本的能力（56.25%）低于LAMP（93.73%）。这可能会影响对感染间日锥虫的牛群进行筛查检测方法的选择。
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