The Key Laboratory for Special Functional Materials of MOE, Henan University, Kaifeng, PR China.
Talanta. 2011 May 15;84(3):777-82. doi: 10.1016/j.talanta.2011.02.004. Epub 2011 Feb 25.
In this work, a highly selective sample cleanup procedure that combining molecular imprinting technique (MIT) and solid phase extraction (SPE) was developed for the isolation of domoic acid (a fascinating marine toxin) from seafood samples. The molecular imprinting polymer (MIP) for domoic acid was prepared using 1,3,5-pentanetricarboxylic acid as the template molecule instead of domoic acid. 4-Vinyl pyridine was used as the functional monomer and ethylene glycol dimethacrylate was used as the cross-linking monomer. The obtained imprinted polymer showed high affinity to domoic acid and was used as selective sorbent for the SPE of domoic acid from seafood samples. An off-line molecularly imprinted solid phase extraction (MISPE) method followed by high-performance liquid chromatography (HPLC) with diode-array detection for the detection of domoic acid was also established. Good linearity was obtained from 0.5 mg L(-1) to 25 mg L(-1) (R(2)>0.99) with a quantitation limit of 0.1 mg L(-1), which was sufficient to determine domoic acid at the maximum level permitted by several authorities. The mean recoveries of domoic acid from mussel extracts were 93.4-96.7%. It was demonstrated that the proposed MISPE-HPLC method could be applied to direct determination of domoic acid from seafood samples.
在这项工作中，开发了一种将分子印迹技术（MIT）和固相萃取（SPE）相结合的高度选择性的样品净化程序，用于从海鲜样品中分离出软骨藻酸（一种引人注目的海洋毒素）。使用 1,3,5-戊烷三羧酸而不是软骨藻酸作为模板分子来制备软骨藻酸的分子印迹聚合物（MIP）。4-乙烯基吡啶用作功能单体，乙二醇二甲基丙烯酸酯用作交联单体。所得印迹聚合物对软骨藻酸具有高亲和力，并用作从海鲜样品中选择性萃取软骨藻酸的选择性吸附剂。还建立了离线分子印迹固相萃取（MISPE）方法，然后用二极管阵列检测高效液相色谱（HPLC）检测软骨藻酸。从 0.5 mg L(-1) 到 25 mg L(-1)（R(2)>0.99）获得了良好的线性关系，定量限为 0.1 mg L(-1)，足以根据几个权威机构的规定确定软骨藻酸的最大允许水平。从贻贝提取物中回收软骨藻酸的平均回收率为 93.4-96.7%。结果表明，所提出的 MISPE-HPLC 方法可用于直接从海鲜样品中测定软骨藻酸。
