Ko Audrey C, Brinton Jason P, Mahajan Vinit B, Zimmerman Bridget, Brinton Gregory S, Stone Edwin M, Folk James C, Mullins Robert F
Department of Ophthalmology and Visual Sciences, University of Iowa, 375 Newton Road, Iowa City, IA 52242, USA.
Arch Ophthalmol. 2011 Apr;129(4):415-20. doi: 10.1001/archophthalmol.2011.65.
To characterize the seroreactivity against retinal proteins in patients with posterior uveitis, retinal disease of noninflammatory origin, and healthy controls.
Patients with posterior uveitis (n = 47), molecularly confirmed photoreceptor degenerations (n = 11), and healthy controls (n = 33) received dilated fundus examinations at the University of Iowa. Aqueous-soluble and detergent-soluble fractions of human retina were separated by gel electrophoresis and transferred to polyvinylidene fluoride membranes. Membranes were probed with patient serum samples to detect IgG, IgA, and IgM human antibodies that react with retinal antigens. The number of bands detected by Western blot was counted, and their molecular weights were determined.
Antibodies recognizing retinal proteins were found in healthy controls, in patients with posterior uveitis, and in patients with molecularly confirmed heritable retinal degenerations. In healthy controls, 42% of individuals had circulating autoantibodies that recognized retinal proteins. Healthy controls had a low odds ratio of serum reactivity to soluble antigens (0.7; 95% confidence interval [CI], 0.4-1.2). Patients with inflammatory retinal diseases and inherited retinal diseases had 4.89 (95% CI, 2.25-10.64; P < .001) and 2.71 (95% CI, 1.19-6.16; P = .02) times more activity against soluble retinal antigens compared with controls.
Healthy control patients exhibited a significantly higher level of background autoantibody activity against retinal proteins than previously reported. Antibody activity in healthy controls was primarily directed against membrane-bound retinal proteins, whereas in patients with pathologic retinal conditions, antibodies targeting nonmembrane-bound retinal proteins predominate.
对后葡萄膜炎患者、非炎性起源的视网膜疾病患者及健康对照者针对视网膜蛋白的血清反应性进行特征描述。
后葡萄膜炎患者（n = 47）、经分子学确诊的光感受器变性患者（n = 11）及健康对照者（n = 33）在爱荷华大学接受散瞳眼底检查。人视网膜的水溶性和去污剂可溶性组分通过凝胶电泳分离，并转移至聚偏二氟乙烯膜上。用患者血清样本对膜进行检测，以检测与视网膜抗原反应的IgG、IgA和IgM人抗体。对通过蛋白质印迹法检测到的条带数量进行计数，并确定其分子量。
在健康对照者、后葡萄膜炎患者及经分子学确诊的遗传性视网膜变性患者中均发现了识别视网膜蛋白的抗体。在健康对照者中，42%的个体具有识别视网膜蛋白的循环自身抗体。健康对照者对可溶性抗原的血清反应性比值比很低（0.7；95%置信区间[CI]，0.4 - 1.2）。与对照相比，炎性视网膜疾病患者和遗传性视网膜疾病患者对可溶性视网膜抗原的活性分别高4.89倍（95% CI，2.25 - 10.64；P <.001）和2.71倍（95% CI，1.19 - 6.16；P = 0.02）。
健康对照患者针对视网膜蛋白的背景自身抗体活性水平显著高于先前报道。健康对照者中的抗体活性主要针对膜结合视网膜蛋白，而在病理性视网膜疾病患者中，针对非膜结合视网膜蛋白的抗体占主导。
