Chambers P J, Saltis J, Alin P, Wright A, Linnane A W, Cheetham B F
Department of Biochemistry, Monash University, Clayton, Victoria, Australia.
Immunopharmacol Immunotoxicol. 1990;12(3):513-25. doi: 10.3109/08923979009006475.
Human interferon alpha 1 (HuIFN alpha 1) is known to protect bovine as well as human cells against viral infection. Hence, we investigated the specificity and tissue distribution of receptors for HuIFN alpha 1 on various cells. [35S]HuIFN alpha 1 bound specifically to homogenates of bovine tissues and particularly to bovine liver, but there was also specific binding to spleen, kidney, brain, adrenal gland, lung, thymus, skeletal muscle, heart, mammary gland and testis. There was no difference in the degree of binding of HuIFN alpha to foetal or adult liver. Competitive binding experiments showed that bovine interferon alpha C (BoIFN alpha C) competed with HuIFN alpha 1 for binding to a bovine liver plasma membrane preparation, indicating that these two IFNs bind to the same receptor. An 35S-labelled IFN alpha 1-receptor complex was isolated from bovine liver extracts by SDS/polyacrylamide gel electrophoresis, and shown to have a molecular weight of 153 kDa. Isolation of the bovine IFN alpha receptor would be a feasible approach to the characterization of the HuIFN alpha receptor.
已知人α1干扰素（HuIFNα1）可保护牛和人细胞免受病毒感染。因此，我们研究了HuIFNα1在各种细胞上的受体特异性和组织分布。[35S]HuIFNα1特异性结合牛组织匀浆，特别是牛肝脏，但也与脾脏、肾脏、大脑、肾上腺、肺、胸腺、骨骼肌、心脏、乳腺和睾丸存在特异性结合。HuIFNα与胎儿或成人肝脏的结合程度没有差异。竞争性结合实验表明，牛αC干扰素（BoIFNαC）与HuIFNα1竞争结合牛肝细胞膜制剂，表明这两种干扰素结合相同的受体。通过SDS/聚丙烯酰胺凝胶电泳从牛肝提取物中分离出35S标记的IFNα1受体复合物，其分子量为153 kDa。分离牛IFNα受体将是表征HuIFNα受体的可行方法。
