College of Life Science, Capital Normal University, Beijing, China.
Biotechnol Prog. 2011 Mar-Apr;27(2):419-27. doi: 10.1002/btpr.545. Epub 2011 Jan 14.
This study reports comparative evaluations of sugar and ethanol production from a native aspen (Populus tremuloides) between sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) and dilute acid (DA) pretreatments. All aqueous pretreatments were carried out in a laboratory wood pulping digester using wood chips at 170°C with a liquid to oven dry (od) wood ratio (L/W) of 3:1 at two levels of acid charge on wood of 0.56 and 1.11%. Sodium bisulfite charge on od wood was 0 for DA and 1.5 or 3.0% for SPORL. All substrates produced by both pretreatments (except DA with pretreatment duration of 0) had good enzymatic digestibility of over 80%. However, SPORL produced higher enzymatic digestibility than its corresponding DA pretreatment for all the experiments conducted. As a result, SPORL produced higher ethanol yield from simultaneous saccharification and fermentation of cellulosic substrate than its corresponding DA pretreatment. SPORL was more effective than its corresponding DA pretreatment in reducing energy consumption for postpretreatment wood chip size-reduction. SPORL, with lower energy input and higher sugar and ethanol yield, produced higher sugar and ethanol production energy efficiencies than the corresponding DA pretreatment.
本研究报告了采用亚硫酸盐预处理克服木质纤维素顽固性（SPORL）和稀酸（DA）预处理对本地白杨（Populus tremuloides）进行糖和乙醇生产的比较评估。所有的水相预处理均在实验室木材制浆蒸煮器中进行，使用 170°C 的木屑，液固比（L/W）为 3:1，酸对木材的负荷为 0.56 和 1.11% 两个水平。DA 预处理的 DA 用量为 0，SPORL 的 DA 用量为 1.5 或 3.0%。两种预处理方法产生的所有底物（DA 预处理时间为 0 的除外）的酶解率均超过 80%。然而，对于所有进行的实验，SPORL 产生的酶解率均高于其对应的 DA 预处理。因此，SPORL 从纤维素底物的同步糖化和发酵中产生的乙醇产率高于其对应的 DA 预处理。SPORL 比其对应的 DA 预处理在降低预处理后木屑尺寸减小的能耗方面更有效。SPORL 具有较低的能耗、更高的糖和乙醇产量，因此其糖和乙醇生产的能量效率高于对应的 DA 预处理。
