Biological Systems Engineering, University of Wisconsin, 460 Henry Mall, Madison, WI 53706, USA.
Bioresour Technol. 2010 May;101(9):3106-14. doi: 10.1016/j.biortech.2009.12.044. Epub 2010 Jan 12.
The performance of two pretreatment methods, sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) and dilute acid (DA), was compared in pretreating softwood (spruce) for fuel ethanol production at 180 degrees Celsius for 30 min with a sulfuric acid loading of 5% on oven-dry wood and a 5:1 liquor-to-wood ratio. SPORL was supplemented with 9% sodium sulfite (w/w of wood). The recoveries of total saccharides (hexoses and pentoses) were 87.9% (SPORL) and 56.7% (DA), while those of cellulose were 92.5% (SPORL) and 77.7% (DA). The total of known inhibitors (furfural, 5-hydroxymethylfurfural, and formic, acetic and levulinic acids) formed in SPORL were only 35% of those formed in DA pretreatment. SPORL pretreatment dissolved approximately 32% of the lignin as lignosulfonate, which is a potential high-value co-product. With an enzyme loading of 15 FPU (filter paper units) per gram of cellulose, the cellulose-to-glucose conversion yields were 91% at 24h for the SPORL substrate and 55% at 48 h for the DA substrate, respectively.
比较了两种预处理方法，亚硫酸盐预处理克服木质纤维素的顽固性（SPORL）和稀酸（DA），在 180°C 下用 5%硫酸载量（基于绝干木材）和 5:1 的液/木比预处理软木（云杉）用于生产燃料乙醇 30 分钟。SPORL 中添加了 9%亚硫酸钠（基于木材的重量）。总糖（己糖和戊糖）的回收率分别为 87.9%（SPORL）和 56.7%（DA），而纤维素的回收率分别为 92.5%（SPORL）和 77.7%（DA）。在 SPORL 中形成的总抑制剂（糠醛、5-羟甲基糠醛、甲酸、乙酸和乙酰丙酸）仅为 DA 预处理中形成的抑制剂的 35%。SPORL 预处理溶解了约 32%的木质素作为木质素磺酸盐，这是一种潜在的高附加值联产产品。在纤维素酶用量为每克纤维素 15FPU（滤纸单位）的情况下，SPORL 底物的纤维素至葡萄糖转化率在 24h 时为 91%，而 DA 底物在 48h 时为 55%。
