Zhang Yan, Wu Yingji
Key Laboratory of China Education Ministry for the Research of Mammal Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot 010021, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2011 Feb;28(1):208-12.
Recent studies have confirmed that diverse adult tissue cells can be reprogrammed and induced to pluripotency, that is so-called induced pluripotent stem cells (iPS cells). But most of these dedifferentiated processes are induced by gene delivery with retroviral vectors. Some of the delivered genes are cancer causing. So, in current situation, these adult-derived embryonic stem-like cells cannot be used in clinical therapy to cure human diseases. Recently some articles that were published in the authoritative journals are receiving attentions. They show that, in mice and human, spermatogonial stem cells (SSCs) can be used for generating pluripotent stem cells without the exogenous genes and retroviruses, and they can also be used for autologous transplantation without ethical problems. These findings suggest that human SSCs may have considerable potential for cell-based, autologous organ regeneration therapy for various diseases. In this review, we describe and compare the methods that have been used to isolate, purificate and culture SSCs. We also describe the recent results in which SSCs can be transformed into pluripotent stem cells, and the pluripotent stem cells have potential applications in regenerative medicine and genetic medicine.
最近的研究证实，多种成体组织细胞可以被重编程并诱导成为多能性细胞，即所谓的诱导多能干细胞（iPS细胞）。但这些去分化过程大多是通过逆转录病毒载体进行基因导入来诱导的。一些导入的基因具有致癌性。所以，在当前情况下，这些源自成体的胚胎干细胞样细胞不能用于临床治疗人类疾病。最近发表在权威期刊上的一些文章受到了关注。它们表明，在小鼠和人类中，精原干细胞（SSCs）无需外源性基因和逆转录病毒即可用于生成多能干细胞，并且它们还可用于自体移植，不存在伦理问题。这些发现表明，人类精原干细胞在基于细胞的各种疾病自体器官再生治疗中可能具有巨大潜力。在这篇综述中，我们描述并比较了用于分离、纯化和培养精原干细胞的方法。我们还描述了精原干细胞可转化为多能干细胞的最新研究结果，以及这些多能干细胞在再生医学和基因医学中的潜在应用。
