National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA.
Biotechniques. 2011 Feb;50(2):96-7. doi: 10.2144/000113600.
Insertion mutagenesis via mobile genetic element is a common technique for the analysis of gene function in model organisms. Next-generation sequencing offers an attractive approach for localizing the site of insertion, but alignment-based mapping of mobile genetic elements is challenging. A computational method for identifying insertion sites is reported herein. The technique was validated by mapping transposons in both bacterial and nematode species. The approach should be extensible to other systems that employ mobile genetic elements to generate mutations.
通过移动遗传元件进行插入诱变是分析模式生物中基因功能的常用技术。下一代测序技术为定位插入位点提供了一种有吸引力的方法，但基于比对的移动遗传元件映射具有挑战性。本文报道了一种用于识别插入位点的计算方法。该技术已通过在细菌和线虫物种中对转座子进行作图验证。该方法应该可以扩展到其他使用移动遗传元件产生突变的系统。
