秀丽隐杆线虫中的正向和反向诱变
Forward and reverse mutagenesis in C. elegans.
作者信息
Kutscher Lena M, Shaham Shai
机构信息
Laboratory of Developmental Genetics, The Rockefeller University, New York NY 10065, USA.
出版信息
WormBook. 2014 Jan 17:1-26. doi: 10.1895/wormbook.1.167.1.
Abstract
Mutagenesis drives natural selection. In the lab, mutations allow gene function to be deciphered. C. elegans is highly amendable to functional genetics because of its short generation time, ease of use, and wealth of available gene-alteration techniques. Here we provide an overview of historical and contemporary methods for mutagenesis in C. elegans, and discuss principles and strategies for forward (genome-wide mutagenesis) and reverse (target-selected and gene-specific mutagenesis) genetic studies in this animal.
摘要
诱变作用推动自然选择。在实验室中，突变有助于解读基因功能。秀丽隐杆线虫因其世代时间短、易于操作且拥有丰富的可用基因改造技术，非常适合进行功能遗传学研究。在此，我们概述了秀丽隐杆线虫诱变的历史和当代方法，并讨论了在该动物中进行正向（全基因组诱变）和反向（目标选择和基因特异性诱变）遗传学研究的原理和策略。
相似文献
1
Forward and reverse mutagenesis in C. elegans.秀丽隐杆线虫中的正向和反向诱变
WormBook. 2014 Jan 17:1-26. doi: 10.1895/wormbook.1.167.1.
2
Reverse genetic strategies in Caenorhabditis elegans: towards controlled manipulation of the genome.秀丽隐杆线虫中的反向遗传策略：迈向基因组的可控操纵
ScientificWorldJournal. 2011 Jul 7;11:1394-410. doi: 10.1100/tsw.2011.126.
3
Harnessing the power of genetics: fast forward genetics in Caenorhabditis elegans.利用遗传学的力量：秀丽隐杆线虫中的快速正向遗传学。
Mol Genet Genomics. 2021 Jan;296(1):1-20. doi: 10.1007/s00438-020-01721-6. Epub 2020 Sep 4.
4
From genes to function: the C. elegans genetic toolbox.从基因到功能：秀丽隐杆线虫的遗传工具箱
Wiley Interdiscip Rev Dev Biol. 2012 Jan-Feb;1(1):114-37. doi: 10.1002/wdev.1. Epub 2011 Nov 28.
5
TILLING is an effective reverse genetics technique for Caenorhabditis elegans.定向诱导基因组局部突变（TILLING）是一种用于秀丽隐杆线虫的有效反向遗传学技术。
BMC Genomics. 2006 Oct 18;7:262. doi: 10.1186/1471-2164-7-262.
6
Genetic analysis of IP3 and calcium signalling pathways in C. elegans.秀丽隐杆线虫中IP3和钙信号通路的遗传分析。
Biochim Biophys Acta. 2012 Aug;1820(8):1253-68. doi: 10.1016/j.bbagen.2011.11.009. Epub 2011 Nov 29.
7
Whole genome sequencing and the transformation of C. elegans forward genetics.全基因组测序与秀丽隐杆线虫正向遗传学的变革。
Methods. 2014 Aug 1;68(3):437-40. doi: 10.1016/j.ymeth.2014.05.008. Epub 2014 May 27.
8
The near demise and subsequent revival of classical genetics for investigating Caenorhabditis elegans embryogenesis: RNAi meets next-generation DNA sequencing.经典遗传学在调查秀丽隐杆线虫胚胎发生过程中的近乎消亡和随后的复兴：RNAi 遇见下一代 DNA 测序。
Mol Biol Cell. 2011 Oct;22(19):3556-8. doi: 10.1091/mbc.E11-03-0185.
9
CRISPR/Cas9-targeted mutagenesis in Caenorhabditis elegans.CRISPR/Cas9 在秀丽隐杆线虫中的靶向突变。
Genetics. 2013 Nov;195(3):1187-91. doi: 10.1534/genetics.113.156299. Epub 2013 Aug 26.
10
Forward and reverse genetics approaches to uncover metabolic aging pathways in Caenorhabditis elegans.利用正向和反向遗传学方法揭示秀丽隐杆线虫代谢衰老途径。
Biochim Biophys Acta Mol Basis Dis. 2018 Sep;1864(9 Pt A):2697-2706. doi: 10.1016/j.bbadis.2017.09.006. Epub 2017 Sep 15.
引用本文的文献
1
Outcrossing Complicates Mutation Purging by Trapping Single Nucleotide Polymorphisms in Structural Variant Mutations.异交通过在结构变异突变中捕获单核苷酸多态性使突变清除变得复杂。
bioRxiv. 2025 Aug 13:2025.04.16.649179. doi: 10.1101/2025.04.16.649179.
2
Dissection of Neurochemical Pathways Across Complexity and Scale.跨越复杂性和尺度的神经化学通路剖析
J Neurochem. 2025 Jul;169(7):e70160. doi: 10.1111/jnc.70160.
3
Cuticular collagens mediate cross-kingdom predator-prey interactions between trapping fungi and nematodes.表皮胶原蛋白介导了捕食性真菌与线虫之间的跨界捕食者 - 猎物相互作用。
PLoS Biol. 2025 Jul 1;23(7):e3003178. doi: 10.1371/journal.pbio.3003178. eCollection 2025 Jul.
4
A targeted genetic screen identifies Caenorhabditis elegans genes involved in RNAi-independent antiviral defense.一项靶向基因筛选鉴定出参与不依赖RNA干扰的抗病毒防御的秀丽隐杆线虫基因。
Virology. 2025 Jun 11;610:110597. doi: 10.1016/j.virol.2025.110597.
5
Brief guide to Caenorhabditis elegans survival assays.秀丽隐杆线虫生存分析简要指南。
Mol Cells. 2025 May 26;48(8):100232. doi: 10.1016/j.mocell.2025.100232.
6
Biased regulation of protein synthesis and hypoxic death by a conditional raptor mutation.通过条件性 Raptor 突变对蛋白质合成和缺氧死亡的偏向性调控
Curr Biol. 2025 Jun 9;35(11):2567-2582.e5. doi: 10.1016/j.cub.2025.04.040. Epub 2025 May 7.
7
Advances in Anthelmintic Target Identification.抗寄生虫药物靶点鉴定的进展
Int J Mol Sci. 2025 Apr 15;26(8):3738. doi: 10.3390/ijms26083738.
8
Conserved chromatin regulators control the transcriptional immune response to intracellular pathogens in Caenorhabditis elegans.保守的染色质调节因子控制秀丽隐杆线虫对细胞内病原体的转录免疫反应。
PLoS Genet. 2025 Apr 7;21(4):e1011444. doi: 10.1371/journal.pgen.1011444. eCollection 2025 Apr.
9
A sequencing-based screening method identifies regulators of EGFR signaling from nonviable mutants in .一种基于测序的筛选方法从……中的非存活突变体中鉴定出表皮生长因子受体（EGFR）信号通路的调节因子。
Sci Signal. 2025 Feb 25;18(875):eadp9377. doi: 10.1126/scisignal.adp9377.
10
A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans.一种通用的定点基因捕获策略，用于操纵秀丽隐杆线虫中的基因活性并控制基因表达。
PLoS Genet. 2025 Jan 22;21(1):e1011541. doi: 10.1371/journal.pgen.1011541. eCollection 2025 Jan.
本文引用的文献
1
The million mutation project: a new approach to genetics in Caenorhabditis elegans.百万突变体项目：秀丽隐杆线虫遗传学的新方法。
Genome Res. 2013 Oct;23(10):1749-62. doi: 10.1101/gr.157651.113. Epub 2013 Jun 25.
2
One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.通过 CRISPR/Cas 介导的基因组工程一步生成携带多个基因突变的小鼠。
Cell. 2013 May 9;153(4):910-8. doi: 10.1016/j.cell.2013.04.025. Epub 2013 May 2.
3
Compact designer TALENs for efficient genome engineering.紧凑型设计的 TALEN 用于高效基因组工程。
Nat Commun. 2013;4:1762. doi: 10.1038/ncomms2782.
4
Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos.利用 RNA 引导的 Cas9 核酸酶在斑马鱼胚胎中进行基因组编辑。
Cell Res. 2013 Apr;23(4):465-72. doi: 10.1038/cr.2013.45. Epub 2013 Mar 26.
5
Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems.利用 CRISPR-Cas 系统进行酿酒酵母的基因组工程。
Nucleic Acids Res. 2013 Apr;41(7):4336-43. doi: 10.1093/nar/gkt135. Epub 2013 Mar 4.
6
Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease.利用 Cas9 RNA 引导的内切酶在人类细胞中进行靶向基因组工程。
Nat Biotechnol. 2013 Mar;31(3):230-2. doi: 10.1038/nbt.2507. Epub 2013 Jan 29.
7
RNA-guided editing of bacterial genomes using CRISPR-Cas systems.利用 CRISPR-Cas 系统对细菌基因组进行 RNA 引导编辑。
Nat Biotechnol. 2013 Mar;31(3):233-9. doi: 10.1038/nbt.2508. Epub 2013 Jan 29.
8
Efficient genome editing in zebrafish using a CRISPR-Cas system.利用 CRISPR-Cas 系统在斑马鱼中进行高效的基因组编辑。
Nat Biotechnol. 2013 Mar;31(3):227-9. doi: 10.1038/nbt.2501. Epub 2013 Jan 29.
9
RNA-guided human genome engineering via Cas9.通过 Cas9 进行 RNA 引导的人类基因组工程。
Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033. Epub 2013 Jan 3.
10
Multiplex genome engineering using CRISPR/Cas systems.利用 CRISPR/Cas 系统进行多重基因组工程。
Science. 2013 Feb 15;339(6121):819-23. doi: 10.1126/science.1231143. Epub 2013 Jan 3.
Zotero 插件