Centre for Phytochemistry and Pharmacology, Southern Cross University, Lismore, NSW, Australia.
Andrologia. 2011 Aug;43(4):273-7. doi: 10.1111/j.1439-0272.2010.01068.x. Epub 2011 Mar 28.
In the present study, the effect of four Vajikaran Rasayana herbs on penile erection, sperm count, seminal fructose content in vivo and nitric oxide (NO) release in vitro was assessed. Lyophilised aqueous extracts of Asparagus racemosus Willd. (AR), Chlorophytum borivilianum Sant. F. (CB), Curculigo orchioides Gaertn. (CO), and Dactylorhiza hatagirea (D. Don) Soo (DH) were orally administered at 100 mg/kg body weight to Wistar strain male albino rats. Penile erection index and sperm count were determined by visual observation; the seminal fructose concentration was measured spectrophotometrically using resorcinol reagent; and NO release was assessed in a mouse macrophage cell line (RAW264) spectrophotometrically using a commercial Griess reagent kit. Penile erection index, sperm count, seminal fructose concentration and in vitro NO release were the parameters measured. A significant effect on the sperm count, seminal fructose content and penile erection index was observed upon treatment with the extracts. The effect of extracts on inducible NO release in vitro directly correlated with the enhanced erectile function in vivo. The aphrodisiac claims attributed to the four Vajikaran Rasayana herbs were tested and a distinctive effect of all extracts tested was observed, with C. borivilianum showing a highly significant response for all parameters measured in vivo and in vitro. The present study also provides a good correlation between the in vivo improvement of penile erection and in vitro NO releasing activity of the extracts. Increase in seminal fructose levels and sperm count further validates the role of these herbs in improving reproductive function.
在本研究中，评估了四种瓦吉卡兰拉沙亚草药对阴茎勃起、精子计数、体内精液果糖含量和体外一氧化氮（NO）释放的影响。将天门冬属植物的冻干水提取物、 Chlorophytum borivilianum 桑特。F.（CB）、 Curculigo orchioides Gaertn.（CO）和 Dactylorhiza hatagirea（D. Don）Soo（DH）以 100mg/kg 体重经口给予 Wistar 品系雄性白化大鼠。通过视觉观察测定阴茎勃起指数和精子计数；使用间苯二酚试剂分光光度法测定精液果糖浓度；使用商业格里斯试剂试剂盒在小鼠巨噬细胞系（RAW264）中分光光度法评估 NO 释放。测量了阴茎勃起指数、精子计数、精液果糖浓度和体外 NO 释放等参数。提取物处理对精子计数、精液果糖含量和阴茎勃起指数有显著影响。提取物对体外诱导型 NO 释放的影响与体内增强的勃起功能直接相关。对四种瓦吉卡兰拉沙亚草药的壮阳功效进行了测试，并观察到所有提取物都有独特的作用，其中 C. borivilianum 在体内和体外测量的所有参数中表现出高度显著的反应。本研究还提供了阴茎勃起的体内改善与提取物体外 NO 释放活性之间的良好相关性。精液果糖水平和精子计数的增加进一步证实了这些草药在改善生殖功能方面的作用。
