Human Sperm Bank, Central South University, Reproductive and Genetic Hospital of Citic Xiangya, Changsha, China.
Andrologia. 2011 Jun;43(3):155-62. doi: 10.1111/j.1439-0272.2009.01036.x. Epub 2011 Feb 25.
The study was aimed at identifying the predictors of the male fertility potential among sperm donors. Fifty anonymous donors undergoing 683 intracervical insemination (ICI) cycles between January 2002 and December 2006 were retrospectively evaluated according to semen characteristics in terms of reproduction rate (RR). We used RR as a parameter to determine the fertility potential among sperm donors. The overall RR was 26.79%. There were no significant differences among low, mean and high RR groups with regard to most sperm routine parameters. However, the RR was notably higher in the sperm morphology of ≥18% than in the <18% group (26.2% versus 19.4% respectively; P < 0.01). Both post-thaw total motility and progressive motility were proportional to RR (P < 0.01). Differences in RR were seen when the percentage of propidium iodide-negative spermatozoa was ≥45% (26.2% versus 16.4% respectively; P < 0.01) and DNA fragmentation index (DFI) was <8% (37.5% versus 17.9% respectively; P < 0.01) in post-thaw samples. Using stepwise linear regression analysis, the percentage of normal morphology, post-thaw progressive motility, PI-negative spermatozoa, DFI had the maximum power to predict the donor fecundity in ICIs.
Both the integrity of plasma membrane and DNA in spermatozoa are crucial factors affecting the fecundity of sperm donors. Therefore, the addition of some of these new tests to routine semen analysis could significantly improve the recruitment of sperm donors and the clinical pregnancy rate of anonymous donors.
研究旨在确定精子供者生育潜能的预测因子。
回顾性分析 2002 年 1 月至 2006 年 12 月间 50 名匿名供者的精液特征，这些供者共接受了 683 次宫腔内人工授精（ICI）周期，根据受孕率（RR）评估其生殖率。我们将 RR 作为参数来确定精子供者的生育潜能。
总的 RR 为 26.79%。在低、中、高 RR 组之间，大多数精子常规参数无显著差异。然而，精子形态≥18%组的 RR 明显高于<18%组（分别为 26.2%和 19.4%；P<0.01）。解冻后总活力和前向运动精子活力均与 RR 成正比（P<0.01）。当解冻后精子中碘化丙啶阴性精子的百分比≥45%（分别为 26.2%和 16.4%；P<0.01）和 DNA 碎片指数（DFI）<8%（分别为 37.5%和 17.9%；P<0.01）时，RR 也存在差异。
通过逐步线性回归分析，正常形态精子百分比、解冻后前向运动精子活力、PI 阴性精子百分比、DFI 对 ICI 中供者生育力的预测能力最大。
精子质膜和 DNA 的完整性是影响精子供者生育力的重要因素。因此，在常规精液分析中增加这些新的检测可以显著提高精子供者的招募和匿名供者的临床妊娠率。
