Marshburn P B, McIntire D, Carr B R, Byrd W
Department of Obstetrics and Gynecology, University of Texas Southwestern Medical Center, Dallas 75235-9032.
Fertil Steril. 1992 Jul;58(1):179-86. doi: 10.1016/s0015-0282(16)55157-7.
To determine if conventional sperm parameters, specific characteristics of sperm motion determined by computer-aided semen analysis (CASA), sperm penetration assay (SPA), and/or spontaneous acrosome reaction assay could best predict fertility outcome after intrauterine insemination (IUI) from frozen donor sperm.
A retrospective analysis of 2,245 cycles of therapeutic donor IUIs were initially studied; 1,147 cycles that met selection criteria were used in this report.
A university-based assisted reproductive technology center.
PATIENTS, PARTICIPANTS: All IUIs were performed on women with documented patency of at least one fallopian tube, ovulatory cycles, and who did not receive human menopausal gonadotropins. Sperm donors had to be used for at least four different recipients (mean of 15) and at least 14 different cycles of insemination (mean of 41).
None.
Pregnancy.
Statistical comparisons were made between donors of different relative fertility by using the Mann-Whitney test, Spearman's rank correlation, and multiple regression analysis. These analyses demonstrated that the most significant predictors of the fertility of frozen-thawed donor sperm were curvilinear velocity, straight line velocity, and the total number of motile sperm inseminated. The number of sperm with spontaneous acrosome reactions negatively correlated with fertility outcome, and SPA provided no predictive value.
Our study supports the hypothesis that the study of sperm motion characteristics using CASA after thawing and washing of cryopreserved sperm is a better predictor of fertile outcome after IUI than analysis of fresh semen.
确定传统的精子参数、计算机辅助精液分析（CASA）测定的精子运动特定特征、精子穿透试验（SPA）和/或自发顶体反应试验能否最好地预测冷冻供体精子宫腔内人工授精（IUI）后的生育结局。
对2245个治疗性供体IUI周期进行回顾性分析，最初进行研究；本报告使用了符合选择标准的1147个周期。
一个大学附属的辅助生殖技术中心。
患者、参与者：所有IUI均针对至少一侧输卵管通畅、有排卵周期且未接受人绝经促性腺激素的女性进行。精子供体必须用于至少四名不同的受者（平均15名）和至少14个不同的授精周期（平均41个）。
无。
妊娠。
采用曼-惠特尼检验、斯皮尔曼等级相关和多元回归分析对不同相对生育力的供体进行统计学比较。这些分析表明，冷冻解冻供体精子生育力的最显著预测因素是曲线速度、直线速度和授精时活动精子的总数。发生自发顶体反应的精子数量与生育结局呈负相关，且SPA无预测价值。
我们的研究支持这样的假设，即与新鲜精液分析相比，对冷冻保存的精子进行解冻和洗涤后使用CASA研究精子运动特征能更好地预测IUI后的生育结局。
