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利用性连锁微卫星标记对九刺鱼（Pungitius pungitius）进行分子性别鉴定和群体遗传推断

Molecular sexing and population genetic inference using a sex-linked microsatellite marker in the nine-spined stickleback (Pungitius pungitius).

作者信息

Shikano Takahito, Herczeg Gábor, Merilä Juha

机构信息

Ecological Genetics Research Unit, Department of Biosciences, University of Helsinki, P,O, Box 65, FI-00014, Helsinki, Finland.

出版信息

BMC Res Notes. 2011 Apr 12;4:119. doi: 10.1186/1756-0500-4-119.

DOI:10.1186/1756-0500-4-119
PMID:21486432
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3094369/
Abstract

BACKGROUND

Sex-specific DNA markers can serve as tools for molecular sex identification, as well as for population genetic inferences. We investigated the potential utility of a microsatellite marker located on sex chromosomes for molecular sexing of Fennoscandian nine-spined sticklebacks (Pungitius pungitius). In addition, we assessed the patterns of allelic differentiation between X and Y chromosomes across the populations to examine if the sex chromosomes had been highly differentiated prior to the postglacial recolonization of Fennoscandia.

FINDINGS

A clear and consistent sex difference in allele size distribution was observed at the Stn19 locus throughout the 15 populations investigated. Males were distinguishable by the presence of distinct male-specific alleles, which were lacking in all females. There was no indication of recombination between sex and the Stn19 locus in the 647 individuals tested. The degree of genetic differentiation between the X and Y chromosomes was much higher than that of interpopulation differentiation in the respective chromosomes.

CONCLUSIONS

Our results indicate that the Stn19 locus can be used for molecular sex identification in Fennoscandian nine-spined sticklebacks. The consistent pattern of high allelic differentiation between the X and Y chromosomes in these populations suggests that the sex chromosomes were already highly differentiated prior to the postglacial recolonization of Fennoscandia.

摘要

背景

性别特异性DNA标记可作为分子性别鉴定以及群体遗传推断的工具。我们研究了位于性染色体上的一个微卫星标记对芬诺斯堪的亚地区九刺鱼（Pungitius pungitius）进行分子性别鉴定的潜在效用。此外，我们评估了各群体中X染色体和Y染色体之间的等位基因分化模式，以检验在芬诺斯堪的亚地区冰期后重新定殖之前，性染色体是否已经高度分化。

研究结果

在所调查的15个群体中，在Stn19位点均观察到等位基因大小分布存在明显且一致的性别差异。雄性可通过存在独特的雄性特异性等位基因来区分，而所有雌性均缺乏这些等位基因。在测试的647个个体中，没有迹象表明性别与Stn19位点之间存在重组。X染色体和Y染色体之间的遗传分化程度远高于各自染色体中的群体间分化程度。

结论

我们的结果表明，Stn19位点可用于芬诺斯堪的亚地区九刺鱼的分子性别鉴定。这些群体中X染色体和Y染色体之间等位基因高度分化的一致模式表明，在芬诺斯堪的亚地区冰期后重新定殖之前，性染色体就已经高度分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a40/3094369/5ca75eeded35/1756-0500-4-119-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a40/3094369/9948000ec3f7/1756-0500-4-119-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a40/3094369/eaaf253c8e00/1756-0500-4-119-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a40/3094369/5ca75eeded35/1756-0500-4-119-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a40/3094369/9948000ec3f7/1756-0500-4-119-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a40/3094369/eaaf253c8e00/1756-0500-4-119-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a40/3094369/5ca75eeded35/1756-0500-4-119-3.jpg

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