Lu Jun-zhang, Zhao Li, Ren Jian-ping, Cao Xiu-tang, Li Hai-xia, Zhao Hong
Department of Traditional Chinese Medicine, Chinese PLA General Hospital, Beijing 100853, China.
Zhong Xi Yi Jie He Xue Bao. 2011 Apr;9(4):435-41. doi: 10.3736/jcim20110413.
To explore the preventive and therapeutic effects of Compound Zhuye Shigao Granule (CZSG), a compound Chinese herbal medicine, on radiation-induced esophagitis in rats.
Fifty-six Wistar rats were randomly divided into 5 groups: normal control group (8 rats), model group (12 rats), and high-, medium- and low-dose CZSG groups (12 rats in each group). The rats in the normal control and model groups were given normal saline 10 mL/kg body weight and those in the CZSG-treated groups were given solution of CZSG at doses of 1.15, 2.30, or 4.60 g/kg body weight respectively by intragastric administration once a day for 7 days. Then esophagitis was induced by local irradiation of (60)Co ray (490.25 cGy/min, totaled 30 Gy). The administration was continued for another 7 days or 14 days, meanwhile body weight and daily food intake of the rats were recorded. Seven days after the irradiation, 4 rats in each group were sacrificed under anesthesia, then, the esophageal tissue was obtained for histopathological examination and the degrees of esophageal tissue injury and neutrophil infiltration were scored. Fourteen days after the irradiation, all the survival rats were dealt in the same way.
(1) Seven days after the irradiation, the esophageal tissue sections in the model group showed typical histopathological changes of radiation-induced esophagitis, whereas in the CZSG groups the histopathological changes were lessened dose-dependently and in the high-dose CZSG group the esophageal tissue remained basically intact. (2) The scores of esophageal tissue injury and cellular infiltration in the high- and medium-dose CZSG groups were both significantly less than in the model group (P<0.05). (3) The body weight of the rats increased in the normal control group, whereas it decreased obviously in the model group. In the medium- and high-dose CZSG groups, it did not decrease significantly. The decrease of body weight in the high-dose CZSG group was less than that in the model group (P<0.05). (4) Compared with the normal control group, the daily food intake was reduced in the other groups. However, it was significantly greater in the low, medium- and high-dose CZSG groups than in the model group (P<0.05).
In rats with radiation-induced esophagitis, CZSG can effectively relieve the esophageal tissue injury and cellular infiltration, increase daily food intake, and prevent rats from lose of body weight dose-dependently, which show that CZSG has the preventive and therapeutic effects for radiation-induced esophagitis in rats.
探讨复方中药制剂复方竹叶石膏颗粒（CZSG）对大鼠放射性食管炎的防治作用。
将56只Wistar大鼠随机分为5组：正常对照组（8只）、模型组（12只）、高、中、低剂量CZSG组（每组12只）。正常对照组和模型组大鼠给予10 mL/kg体重的生理盐水，CZSG处理组大鼠分别给予剂量为1.15、2.30或4.60 g/kg体重的CZSG溶液，每天灌胃1次，连续7天。然后通过局部照射（60）Co射线（490.25 cGy/min，总量30 Gy）诱导食管炎。给药再持续7天或14天，同时记录大鼠的体重和每日食物摄入量。照射7天后，每组麻醉处死4只大鼠，取食管组织进行组织病理学检查，对食管组织损伤程度和中性粒细胞浸润进行评分。照射14天后，对所有存活大鼠进行同样处理。
（1）照射7天后，模型组食管组织切片显示出典型的放射性食管炎组织病理学变化，而CZSG组组织病理学变化呈剂量依赖性减轻，高剂量CZSG组食管组织基本保持完整。（2）高、中剂量CZSG组食管组织损伤和细胞浸润评分均显著低于模型组（P<0.05）。（3）正常对照组大鼠体重增加，而模型组大鼠体重明显下降。中、高剂量CZSG组大鼠体重无明显下降。高剂量CZSG组大鼠体重下降幅度小于模型组（P<0.05）。（4）与正常对照组相比，其他组大鼠每日食物摄入量减少。然而，低、中、高剂量CZSG组大鼠每日食物摄入量明显高于模型组（P<0.05）。
在放射性食管炎大鼠中，CZSG可有效减轻食管组织损伤和细胞浸润，增加每日食物摄入量，并剂量依赖性地防止大鼠体重减轻，表明CZSG对大鼠放射性食管炎具有防治作用。
