Lu Jun-zhang, Wang Chen, Yang Ming-hui, Zhao Hong, Liu Yi, Cao Xiu-tang
Department of Chinese Medicine, Chinese PLA General Hospital, Beijing, 100853, China,
Chin J Integr Med. 2014 Jun;20(6):462-7. doi: 10.1007/s11655-014-1754-1. Epub 2014 Jun 21.
To investigate the effect of Modified Zhuye Shigao Decoction (MZSD) and its components on preventing radiation esophagitis of rats.
One hundred Wistar rats were randomly divided into 5 groups, including the control group, radiation model group, MZSD group, Zhuye Shigao Decoction (ZSD) group, and added ingredients group, 20 rats in each group. The model of radiation esophagitis of rat was established by once local radiation of 40 Gy (330 Mu/min) with a high energy linear accelerator. The administration of Chinese medicine was continued for 14 days from 7 days before radiation application in the three treatment groups. On the 7th and 14th day, the serum was isolated and the levels of inflammatory cytokines tumor necrosis factor (TNF-α), interleukin 1β (IL-1β) and IL-8 were tested. The pathological slices of esophagus were obtained, and the pathological changes were observed. During the whole process, weight and food intake were recorded each day.
On the 7th day after radiation, the esophagus of rats in the MZSD group was almost intact, and the pathological injury score was significantly lower than that of the radiation model group, ZSD group and added ingredients group (P<0.01). Compared with the control group, the body weight and food intake of rats in the radiation model group were significantly decreased, and the levels of TNF-α, IL-1β and IL-8 were significantly increased (P<0.05 or P<0.01), while the MZSD group showed a significant increase in body weight and food intake, and a significant decrease in the levels of TNF-α, IL-1β and IL-8 compared with the radiation model group, ZSD group and added ingredients group (P <0.05 or P<0.01).
MZSD prevents the development of radiation esophagitis probably by inhibiting the generation and release of the inflammatory cytokines TNF-α, IL-1β and IL-8.
探讨加味竹叶石膏汤及其各组分对大鼠放射性食管炎的预防作用。
将100只Wistar大鼠随机分为5组，即对照组、辐射模型组、加味竹叶石膏汤组、竹叶石膏汤组和加味组，每组20只。采用高能直线加速器一次性局部照射40 Gy（330 毫戈瑞/分钟）建立大鼠放射性食管炎模型。三个治疗组于照射前7天开始连续灌胃给药14天。于照射后第7天和第14天分离血清，检测炎症细胞因子肿瘤坏死因子（TNF-α）、白细胞介素1β（IL-1β）和IL-8水平。取食管病理切片，观察病理变化。整个过程中每天记录大鼠体重和进食量。
照射后第7天，加味竹叶石膏汤组大鼠食管基本完整，病理损伤评分显著低于辐射模型组、竹叶石膏汤组和加味组（P<0.01）。与对照组比较，辐射模型组大鼠体重和进食量显著降低，TNF-α、IL-1β和IL-8水平显著升高（P<0.05或P<0.01）；与辐射模型组、竹叶石膏汤组和加味组比较，加味竹叶石膏汤组大鼠体重和进食量显著增加，TNF-α、IL-1β和IL-8水平显著降低（P<0.05或P<0.01）。
加味竹叶石膏汤可能通过抑制炎症细胞因子TNF-α、IL-1β和IL-8的产生与释放来预防放射性食管炎的发生。
