Owoyele Bamidele Victor, Adenekan Omosunkanmi Toyin, Soladoye Ayodele Olufemi
Department of Physiology, University of Ilorin, Ilorin, Nigeria.
Zhong Xi Yi Jie He Xue Bao. 2011 Apr;9(4):447-52. doi: 10.3736/jcim20110415.
The aim of the study is to investigate the effects of honey on acute and chronic inflammations and nitric oxide production in rats.
Carrageenan, cotton pellet and formaldehyde methods were used in quantifying the anti-inflammatory effect of honey while the effect of honey on nitric oxide (NO) production was investigated by administering NG-nitro-L-arginine methyl ester (L-NAME, 100 mg/kg body weight, subcutaneously) and L-arginine (300 mg/kg body weight, intraperitoneally) to groups of rats. Animals were divided into five groups each comprising of five rats in each experiment; two groups were orally administered distilled water (control) and indomethacin (5 mg/kg body weight), respectively, while the remaining three groups were administered 2, 6 and 10 g/kg honey for anti-inflammatory studies.
Honey significantly (P<0.05) reduced the paw size in the carrageenan model, while in the cotton pellet model, the granuloma weight was significantly (P<0.05) reduced. Honey also significantly (P<0.05) reduced the arthritis induced by formaldehyde injection from the second day of the study. In the investigation on NO involvement, L-NAME significantly inhibited paw oedema while the administration of L-arginine abolished the anti-inflammatory effect of honey and L-NAME.
The results obtained from the study confirm that honey has an anti-inflammatory effect which may be due in part to inhibition of NO release. Therefore honey may be used to treat certain acute and chronic inflammatory conditions.
本研究旨在探讨蜂蜜对大鼠急性和慢性炎症以及一氧化氮生成的影响。
采用角叉菜胶、棉球和甲醛法来量化蜂蜜的抗炎作用，同时通过给大鼠皮下注射NG-硝基-L-精氨酸甲酯（L-NAME，100毫克/千克体重）和腹腔注射L-精氨酸（300毫克/千克体重）来研究蜂蜜对一氧化氮（NO）生成的影响。在每个实验中，将动物分为五组，每组五只大鼠；两组分别口服蒸馏水（对照组）和吲哚美辛（5毫克/千克体重），而其余三组分别给予2、6和10克/千克蜂蜜进行抗炎研究。
在角叉菜胶模型中，蜂蜜显著（P<0.05）减小了爪部尺寸，而在棉球模型中，肉芽肿重量显著（P<0.05）减轻。从研究的第二天起，蜂蜜还显著（P<0.05）减轻了甲醛注射诱发的关节炎。在关于NO参与的研究中，L-NAME显著抑制了爪部水肿，而L-精氨酸的给药消除了蜂蜜和L-NAME的抗炎作用。
本研究获得的结果证实，蜂蜜具有抗炎作用，这可能部分归因于对NO释放的抑制。因此，蜂蜜可用于治疗某些急性和慢性炎症性疾病。
