College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Res Vet Sci. 2011 Dec;91(3):e16-24. doi: 10.1016/j.rvsc.2011.02.015. Epub 2011 Apr 12.
The present work describes the distribution of S-100 protein in the intestinal tract of a Chinese soft-shelled turtle specimen (Pelodiscus sinensis). S-100 protein positive cells were located in the intestinal tract, from the proximal small to distal large intestine. S-100 protein positive dendritic cells had irregular shape and were positive in both cytoplasm and nucleus. Most of them were located both lamina propria and submucosa in the small intestine, while few were found in the large intestine. S-100 protein, C-kit positive ICCs and Silver staining glial cells were predominantly observed in three locations: (1) in the interspace between the submucosa and circular muscle layer; (2) in the circular muscle layer; and (3) between the circular and longitudinal muscle layers of the intestine. Fewer were found in the large intestinal lamina propria and submucosa. Three types of positive cells (S-100 protein positive cells, C-kit positive ICCs and Silver staining glial cells) with 1-2 long or 2-3 short processes were distributed as lace-like or surrounding blood vessels in the different locations mentioned above. In the lamina propria, all the positive cells with irregular processes were connected with each other and formed a network. In the submucosa, all the positive cells were found surrounding the blood vessels.
本研究描述了中国鳖（Pelodiscus sinensis）肠道中 S-100 蛋白的分布。S-100 蛋白阳性细胞位于肠道，从小肠近端到大肠远端。S-100 蛋白阳性树突状细胞形状不规则，胞质和胞核均呈阳性。它们大多位于小肠固有层和黏膜下层，而在大肠中则很少见。S-100 蛋白、C-kit 阳性 ICC 和银染神经胶质细胞主要观察到三个部位：（1）黏膜下层和环形肌层之间的间隙；（2）环形肌层；（3）肠的环形和纵形肌层之间。在大肠固有层和黏膜下层中发现的数量较少。在上述不同部位，具有 1-2 个长或 2-3 个短突起的 3 种阳性细胞（S-100 蛋白阳性细胞、C-kit 阳性 ICC 和银染神经胶质细胞）呈花边状或围绕血管分布。在固有层，所有具有不规则突起的阳性细胞相互连接形成网络。在黏膜下层，所有的阳性细胞都围绕着血管。
