Miralpeix M, Katz N R, Bartrons R
Departament de Ciències Fisiològiques Humanes de la Nutrició, Universitat de Barcelona, Spain.
Cell Biochem Funct. 1990 Oct;8(4):237-41. doi: 10.1002/cbf.290080409.
The presence of vanadate in primary cultures of rat hepatocytes produced a significant increase in the concentration of fructose 2,6-bisphosphate and in the activity of 6-phosphofructo 2-kinase. Compared with insulin, vanadate had a more potent action on the metabolite increase, but a similar effect on the 6-phosphofructo 2-kinase activity. Both the insulin- and the vanadate-dependent enhancements of 6-phosphofructo 2-kinase were inhibited by cycloheximide which specifically blocks protein synthesis on the translational level, suggesting that the increase of the enzyme activity was due to induction rather than to a change in the catalytic activity.
钒酸盐存在于大鼠肝细胞原代培养物中时，会使果糖2,6 -二磷酸的浓度以及6 -磷酸果糖-2 -激酶的活性显著增加。与胰岛素相比，钒酸盐对代谢物增加的作用更强，但对6 -磷酸果糖-2 -激酶活性的影响相似。胰岛素和钒酸盐依赖的6 -磷酸果糖-2 -激酶增强作用均被环己酰亚胺抑制，环己酰亚胺在翻译水平特异性阻断蛋白质合成，这表明酶活性的增加是由于诱导作用而非催化活性的改变。
