Protein Engineering and Proteomics Group, Department of Chemistry, Biotechnology and Food Sciences, Norwegian University of Life Sciences, Ås, 1430, Norway.
Appl Biochem Biotechnol. 2011 Sep;165(1):204-20. doi: 10.1007/s12010-011-9244-9. Epub 2011 Apr 13.
In solid-state fermentation, among various solid supports evaluated, banana peel was found to be an ideal support and resulted into higher levels of laccase (6281.4 ± 63.60 U l(-1)) along with notable levels of manganese peroxidase production (1339.0 ± 131.23 U l(-1)) by Aspergillus fumigatus VkJ2.4.5. Maximum levels of laccase was achieved under derived conditions consisting of 80% of moisture level, 6 days of incubation period, 6% inoculum level, and an aeration level of 2.5 l min(-1). A column-tray bioreactor was designed to scale up and economize the enzyme production in three successive cycles of fermentation using the same fungal biomass. Thermal and pH stability profiles revealed that enzyme was stable up to 50°C and at varying pH range from 5-9 for up to 2 h. The apparent molecular weight of laccase was found to be 34 ± 1 kDa. MALDI-TOF/TOF analysis of the protein showed significant homology with maximum identity of 67% to other laccases reported in database.
在固态发酵中，在所评估的各种固体载体中，发现香蕉皮是一种理想的载体，可使烟曲霉 VkJ2.4.5 产生更高水平的漆酶（6281.4 ± 63.60 U l（-1））和显著水平的锰过氧化物酶（1339.0 ± 131.23 U l（-1））。在衍生条件下，漆酶的最高水平达到了 80%的水分水平、6 天的培养期、6%的接种水平和 2.5 l min（-1）的通气水平。设计了一个柱式托盘生物反应器，以相同的真菌生物量在三个连续的发酵循环中扩大规模并节约酶的生产。热和 pH 稳定性谱表明，酶在 50°C 以下和 pH 范围从 5 到 9 下保持稳定长达 2 小时。漆酶的表观分子量为 34 ± 1 kDa。MALDI-TOF/TOF 分析表明，该蛋白与数据库中报道的其他漆酶具有显著的同源性，最大同一性为 67%。
