Division of Physical Therapy, Georgia State University, P.O. Box 4019, Atlanta, Georgia 30302, USA.
Muscle Nerve. 2011 Jul;44(1):85-92. doi: 10.1002/mus.22001. Epub 2011 Apr 12.
Many aspects of skeletal muscle regeneration are now considered to be controlled by the innate immune system, specifically macrophages, but the mechanisms for activation and modulation of the innate immune system during injury are not well understood.
We analyzed the expression of toll-like receptors (TLRs) and adenosine receptors during traumatic skeletal muscle injury. mRNA expression and immunostaining of these receptors were evaluated in mouse skeletal muscle injured by freezing.
Expression of nearly all mammalian TLRs was induced at 1 and/or 3 days postinjury with a common trend for higher expression at day 3. Injury also elicited a dramatic increase in the expression of adenosine receptors A(2B) and A(3) but not A(1) and A(2A) .
Both receptor types may be potential targets for stimulation of skeletal muscle tissue regeneration and functional restoration after injury.
现在人们认为，骨骼肌肉再生的许多方面都受到固有免疫系统（尤其是巨噬细胞）的控制，但对于损伤过程中固有免疫系统的激活和调节机制，人们还知之甚少。
我们分析了创伤性骨骼肌肉损伤过程中 toll 样受体（TLRs）和腺苷受体的表达情况。通过冷冻法损伤小鼠的骨骼肌肉，评估这些受体的 mRNA 表达和免疫染色情况。
几乎所有哺乳动物 TLR 的表达在损伤后 1 天和/或 3 天被诱导，第 3 天的表达趋势更高。损伤还引起了腺苷受体 A(2B)和 A(3)的表达显著增加，但 A(1)和 A(2A)的表达没有增加。
这两种受体类型可能是损伤后刺激骨骼肌肉组织再生和功能恢复的潜在靶点。
