Tapia-Hernández A, Mascarúa-Esparza M A, Caballero-Mellado J
Departamento de Investigaciones Microbiológicas, Universidad Autónoma de Puebla, México.
Microbios. 1990;64(259):73-83.
Sixty Azospirillum strains were tested for their bacteriocin production ability; twenty-seven (45%) were able to produce bacteriocins and inhibited the growth of one or more indicator strains in solid medium. Mitomycin C treatment enhanced the proportion to 80%. Sometimes large growth inhibition zones were formed, but not when FeCl3 was added in the medium. These inhibition zones probably result from the activity of siderophores. Partially purified bacteriocins produced by four strains were inactivated at pH 4, but were very stable between pH 5 to 10; bacteriocins produced by three strains lost their activity between 55 and 80 degrees C. Loss or decrease in the bacteriocin activity was observed with pronase E treatment; trypsin, lysozyme and alpha-amylase did not have an effect on bacteriocin activity. These findings show that the antagonism among azospirilla was due principally to the bacteriocins and sometimes probably due to siderophores, but not to bacteriophages or other substances.
对60株固氮螺菌菌株进行了细菌素产生能力测试；其中27株（45%）能够产生细菌素，并在固体培养基中抑制一种或多种指示菌株的生长。丝裂霉素C处理可将这一比例提高到80%。有时会形成较大的生长抑制区，但在培养基中添加FeCl3时则不会。这些抑制区可能是铁载体活性的结果。四株菌株产生的部分纯化细菌素在pH 4时失活，但在pH 5至10之间非常稳定；三株菌株产生的细菌素在55至80摄氏度之间失去活性。经链霉蛋白酶E处理后，细菌素活性丧失或降低；胰蛋白酶、溶菌酶和α淀粉酶对细菌素活性没有影响。这些发现表明，固氮螺菌之间的拮抗作用主要归因于细菌素，有时可能归因于铁载体，但不是噬菌体或其他物质。
