College of Life and Environment Sciences, Shanghai Normal University, Guilin Road 100, Shanghai 200234, China.
Bioresour Technol. 2011 Sep;102(18):8635-8. doi: 10.1016/j.biortech.2011.03.052. Epub 2011 Mar 22.
Treatment with NaHSO3 induces a 10-fold increase in H2 photoproduction in the filamentous N2-fixing cyanobacterium Anabaena sp. strain PCC 7120. However, it is unclear whether this treatment also increases H2 photoproduction in green alga. In this study, treatment with 13 mM NaHSO3 resulted in about a 200-fold increase in H2 production in Chlamydomonas reinhardtii, and this increase was most probably the result of reduced O2 content and enhanced hydrogenase activity. Compared to the conventional strategy of sulfur deprivation, NaHSO3 treatment results in a higher maximum rate of H2 photoproduction, greater efficiency of conversion of light energy into H2, shorter half-time to produce the maximum accumulated H2 levels, and reduced costs because no centrifugation is involved. We therefore conclude that NaHSO3 treatment is an efficient, rapid, and economic strategy for improving photobiological H2 production in the green alga C. reinhardtii.
亚硫酸氢钠处理可使固氮丝状蓝藻鱼腥藻 PCC 7120 的氢气光产量增加 10 倍。然而，目前尚不清楚这种处理是否也能增加绿藻的氢气光产量。在本研究中，用 13 mM 的亚硫酸氢钠处理莱茵衣藻，可使氢气产量增加约 200 倍，这很可能是由于氧含量降低和氢化酶活性增强所致。与传统的硫剥夺策略相比，亚硫酸氢钠处理可使氢气光生产的最大速率更高，光能转化为氢气的效率更高，达到最大累积氢气水平的半衰期更短，且成本更低，因为无需离心。因此，我们得出结论，亚硫酸氢钠处理是提高绿藻莱茵衣藻生物光制氢的一种有效、快速且经济的策略。
