Wei Lanzhen, Yi Jing, Wang Lianjun, Huang Tingting, Gao Fudan, Wang Quanxi, Ma Weimin
Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
College of Life Sciences, South China Agricultural University, Guangzhou, China.
Plant Cell Physiol. 2017 Mar 1;58(3):451-457. doi: 10.1093/pcp/pcw216.
Chlamydomonas reinhardtii is a unicellular green alga that can use light energy to produce H2 from H2O in the background of NaHSO3 treatment. However, the role of light intensity in such H2 production remains elusive. Here, light intensity significantly affected the yield of H2 production in NaHSO3-treated C. reinhardtii, which was consistent with its effects on the content of O2 and the expression and activity of hydrogenase. Further, NaHSO3 was found to be able to remove O2 via a reaction of bisulfite with superoxide anion produced at the acceptor side of PSI, and light intensity affected the reaction rate significantly. Accordingly, high light and strong light but not low light can create an anaerobic environment, which is important to activate hydrogenase and produce H2. Based on the above results, we conclude that light intensity plays an important role in removing O2 and consequently activating hydrogenase and producing H2 in NaHSO3-treated C. reinhardtii.
莱茵衣藻是一种单细胞绿藻，在亚硫酸氢钠处理的背景下，它能够利用光能从水中产生氢气。然而，光强在这种产氢过程中的作用仍不清楚。在此，光强显著影响了亚硫酸氢钠处理的莱茵衣藻的产氢量，这与其对氧气含量以及氢化酶的表达和活性的影响相一致。此外，发现亚硫酸氢钠能够通过亚硫酸氢盐与光合系统I受体侧产生的超氧阴离子的反应来去除氧气，并且光强对该反应速率有显著影响。因此，强光和较强光而非弱光能够创造一个厌氧环境，这对于激活氢化酶和产生氢气很重要。基于上述结果，我们得出结论，光强在亚硫酸氢钠处理的莱茵衣藻中对于去除氧气进而激活氢化酶和产生氢气起着重要作用。
