咽拭子在检测下呼吸道感染住院患儿病毒中的附加价值。
Added value of an oropharyngeal swab in detection of viruses in children hospitalized with lower respiratory tract infection.
机构信息
KEMRI Wellcome Trust Research Programme, Kilifi, Kenya.
出版信息
J Clin Microbiol. 2011 Jun;49(6):2318-20. doi: 10.1128/JCM.02605-10. Epub 2011 Apr 13.
Abstract
Paired nasopharyngeal and oropharyngeal swabs collected from 533 children hospitalized with lower respiratory tract infection were assessed by multiplex reverse transcription-PCR. Oropharyngeal swabs increased the number of viral infections detected by 15%, compared to collection of a nasopharyngeal swab alone. This advantage was most pronounced for detection of influenza, parainfluenza, and adenovirus.
摘要
对 533 例因下呼吸道感染住院的儿童进行了检测，使用多重逆转录-PCR 对采集的鼻咽和口咽拭子进行分析。与单独采集鼻咽拭子相比，采集口咽拭子将病毒感染的检出数量增加了 15%。这一优势在流感、副流感和腺病毒的检测中最为显著。
相似文献
1
Added value of an oropharyngeal swab in detection of viruses in children hospitalized with lower respiratory tract infection.咽拭子在检测下呼吸道感染住院患儿病毒中的附加价值。
J Clin Microbiol. 2011 Jun;49(6):2318-20. doi: 10.1128/JCM.02605-10. Epub 2011 Apr 13.
2
What Is the Added Benefit of Oropharyngeal Swabs Compared to Nasal Swabs Alone for Respiratory Virus Detection in Hospitalized Children Aged <10 Years?对于 10 岁以下住院儿童，与单独使用鼻咽拭子相比，口咽拭子在呼吸道病毒检测方面的额外益处是什么？
J Infect Dis. 2015 Nov 15;212(10):1600-3. doi: 10.1093/infdis/jiv265. Epub 2015 May 5.
3
Comparison of nasopharyngeal flocked swabs and nasopharyngeal wash collection methods for respiratory virus detection in hospitalized children using real-time polymerase chain reaction.比较实时聚合酶链反应检测住院儿童呼吸道病毒时使用的鼻咽拭子和鼻咽洗液采集方法。
J Virol Methods. 2012 Oct;185(1):89-93. doi: 10.1016/j.jviromet.2012.06.009. Epub 2012 Jun 21.
4
Comparison of nasopharyngeal and oropharyngeal swabs for the diagnosis of eight respiratory viruses by real-time reverse transcription-PCR assays.实时逆转录聚合酶链反应检测鼻咽拭子和口咽拭子对 8 种呼吸道病毒的诊断比较。
PLoS One. 2011;6(6):e21610. doi: 10.1371/journal.pone.0021610. Epub 2011 Jun 30.
5
Detection of respiratory viruses by PCR assay of nasopharyngeal swabs stored in skim milk-tryptone-glucose-glycerol transport medium.应用 PCR 法检测保存在脱脂乳-胰蛋白胨-葡萄糖-甘油运输培养基中的鼻咽拭子中的呼吸道病毒。
J Clin Microbiol. 2011 Jun;49(6):2311-3. doi: 10.1128/JCM.00224-11. Epub 2011 Mar 30.
6
Rapid detection of respiratory tract viral infections and coinfections in patients with influenza-like illnesses by use of reverse transcription-PCR DNA microarray systems.应用逆转录-PCR DNA 微阵列系统快速检测流感样疾病患者的呼吸道病毒感染及合并感染。
J Clin Microbiol. 2010 Nov;48(11):3836-42. doi: 10.1128/JCM.00733-10. Epub 2010 Aug 25.
7
Comparison of nasopharyngeal flocked swabs and aspirates for rapid diagnosis of respiratory viruses in children.鼻咽部植绒拭子与吸取物用于儿童呼吸道病毒快速诊断的比较
J Clin Virol. 2008 May;42(1):65-9. doi: 10.1016/j.jcv.2007.12.003. Epub 2008 Feb 1.
8
Pooled nasopharyngeal and oropharyngeal samples for the identification of respiratory viruses in adults.用于鉴定成人呼吸道病毒的鼻咽和口咽合并样本。
Eur J Clin Microbiol Infect Dis. 2010 Jun;29(6):733-5. doi: 10.1007/s10096-010-0903-5. Epub 2010 Mar 12.
9
Improved detection of respiratory viruses in pediatric outpatients with acute respiratory illness by real-time PCR using nasopharyngeal flocked swabs.实时荧光 PCR 法采用鼻咽拭子采集标本检测儿科门诊急性呼吸道感染患儿呼吸道病毒的效果研究
J Clin Microbiol. 2011 Sep;49(9):3365-7. doi: 10.1128/JCM.02231-10. Epub 2011 Jul 20.
10
Nasal swab versus nasopharyngeal aspirate for isolation of respiratory viruses.用于分离呼吸道病毒的鼻拭子与鼻咽抽吸物比较
J Clin Microbiol. 2002 Nov;40(11):4337-9. doi: 10.1128/JCM.40.11.4337-4339.2002.
引用本文的文献
1
Molecular Analysis of Human Respiratory Syncytial Virus Group B Strains Isolated in Kenya Before and During the Emergence of Pandemic Influenza A/H1N1.甲型H1N1流感大流行出现之前及期间在肯尼亚分离出的人呼吸道合胞病毒B组毒株的分子分析
Influenza Other Respir Viruses. 2025 Feb;19(2):e70082. doi: 10.1111/irv.70082.
2
Local patterns of spread of influenza A H3N2 virus in coastal Kenya over a 1-year period revealed through virus sequence data.在肯尼亚沿海地区，通过病毒序列数据揭示了 A 型 H3N2 流感病毒在 1 年内的局部传播模式。
Sci Rep. 2024 Oct 8;14(1):23426. doi: 10.1038/s41598-024-74218-6.
3
Spatio-temporal distribution of rhinovirus types in Kenya: a retrospective analysis, 2014.肯尼亚鼻病毒类型的时空分布：一项回顾性分析，2014 年。
Sci Rep. 2024 Sep 27;14(1):22298. doi: 10.1038/s41598-024-73856-0.
4
The cost of care for children hospitalized with respiratory syncytial virus (RSV) associated lower respiratory infection in Kenya.肯尼亚因呼吸道合胞病毒（RSV）相关下呼吸道感染而住院的儿童的护理成本。
BMC Public Health. 2024 Sep 4;24(1):2410. doi: 10.1186/s12889-024-19875-y.
5
Enrichment approach for unbiased sequencing of respiratory syncytial virus directly from clinical samples.直接从临床样本中对呼吸道合胞病毒进行无偏差测序的富集方法。
Wellcome Open Res. 2021 May 7;6:99. doi: 10.12688/wellcomeopenres.16756.1. eCollection 2021.
6
Clinical Performance of Self-Collected Purified Water Gargle for Detection of Influenza a Virus Infection by Real-Time RT-PCR.通过实时逆转录聚合酶链反应检测甲型流感病毒感染时，自行采集的纯化水漱口液的临床性能
Infect Drug Resist. 2024 May 14;17:1903-1910. doi: 10.2147/IDR.S450991. eCollection 2024.
7
Rhinovirus dynamics across different social structures.不同社会结构中的鼻病毒动态变化。
Npj Viruses. 2023;1(1):6. doi: 10.1038/s44298-023-00008-y. Epub 2023 Nov 27.
8
Unique microbial landscape in the human oropharynx during different types of acute respiratory tract infections.人类口咽部不同类型急性呼吸道感染期间的独特微生物景观。
Microbiome. 2023 Jul 24;11(1):157. doi: 10.1186/s40168-023-01597-9.
9
cGAS and DDX41-STING mediated intrinsic immunity spreads intercellularly to promote neuroinflammation in SOD1 ALS model.cGAS和DDX41-STING介导的固有免疫在细胞间传播，以促进SOD1肌萎缩侧索硬化模型中的神经炎症。
iScience. 2022 May 13;25(6):104404. doi: 10.1016/j.isci.2022.104404. eCollection 2022 Jun 17.
10
Genome-scale CRISPR screens identify host factors that promote human coronavirus infection.全基因组 CRISPR 筛选鉴定出促进人类冠状病毒感染的宿主因子。
Genome Med. 2022 Jan 27;14(1):10. doi: 10.1186/s13073-022-01013-1.
本文引用的文献
1
Comparison of midturbinate flocked-swab specimens with nasopharyngeal aspirates for detection of respiratory viruses in children by the direct fluorescent antibody technique.应用直接免疫荧光法比较鼻内拭子和鼻咽抽吸物在儿童呼吸道病毒检测中的应用。
J Clin Microbiol. 2010 Oct;48(10):3742-3. doi: 10.1128/JCM.01520-10. Epub 2010 Aug 11.
2
Pooled nasopharyngeal and oropharyngeal samples for the identification of respiratory viruses in adults.用于鉴定成人呼吸道病毒的鼻咽和口咽合并样本。
Eur J Clin Microbiol Infect Dis. 2010 Jun;29(6):733-5. doi: 10.1007/s10096-010-0903-5. Epub 2010 Mar 12.
3
Clinical performance of a rapid influenza test and comparison of nasal versus throat swabs to detect 2009 pandemic influenza A (H1N1) infection in Thai children.一种快速流感检测的临床性能，以及比较鼻拭子和咽拭子在检测泰国儿童 2009 年甲型 H1N1 流感感染中的应用。
Pediatr Infect Dis J. 2010 Apr;29(4):366-7. doi: 10.1097/INF.0b013e3181c6f05c.
4
Identification of respiratory viruses in adults: nasopharyngeal versus oropharyngeal sampling.成人呼吸道病毒的检测：鼻咽与口咽采样比较。
J Clin Microbiol. 2009 Nov;47(11):3439-43. doi: 10.1128/JCM.00886-09. Epub 2009 Sep 2.
5
Comparison between pernasal flocked swabs and nasopharyngeal aspirates for detection of common respiratory viruses in samples from children.经鼻植绒拭子与鼻咽抽吸物在检测儿童样本中常见呼吸道病毒方面的比较。
J Clin Microbiol. 2008 Jul;46(7):2414-7. doi: 10.1128/JCM.00369-08. Epub 2008 May 14.
6
Comparison of respiratory virus detection rates for infants and toddlers by use of flocked swabs, saline aspirates, and saline aspirates mixed in universal transport medium for room temperature storage and shipping.使用植绒拭子、盐水抽吸物以及在通用运输培养基中混合的盐水抽吸物进行室温储存和运输时，婴幼儿呼吸道病毒检测率的比较。
J Clin Microbiol. 2008 Jul;46(7):2374-6. doi: 10.1128/JCM.00714-08. Epub 2008 May 7.
7
Use of throat swab or saliva specimens for detection of respiratory viruses in children.使用咽拭子或唾液标本检测儿童呼吸道病毒。
Clin Infect Dis. 2008 Apr 1;46(7):e61-4. doi: 10.1086/529386.
8
Three Indonesian clusters of H5N1 virus infection in 2005.2005年印度尼西亚的三起H5N1病毒感染群聚事件。
N Engl J Med. 2006 Nov 23;355(21):2186-94. doi: 10.1056/NEJMoa060930.
9
Real-time RT-PCR detection of 12 respiratory viral infections in four triplex reactions.在四个三重反应中对12种呼吸道病毒感染进行实时逆转录聚合酶链反应检测。
J Clin Virol. 2005 Aug;33(4):341-4. doi: 10.1016/j.jcv.2004.11.025.
Zotero 插件