Denker P S, Vesely D L, Gómez-Sánchez C E
Department of Internal Medicine, Univeristy of South Florida Health Sciences Center, Tampa.
J Steroid Biochem Mol Biol. 1990 Nov 30;37(4):617-9. doi: 10.1016/0960-0760(90)90410-m.
Pro-atrial natriuretic peptide (Pro-ANP) is a 126 amino acid peptide from which atrial natriuretic peptide (ANP) (99-126 amino acid) is derived. ANP has potent diuretic, natriuretic and vasodilatory peptides. ANP is also a potent direct and indirect inhibitor of aldosterone secretion. The N-terminus of the ANP prohormone containing the peptides 1-30 and 31-67 have been demonstrated to have diuretic, natriuretic and vasodilatory properties. Dispersed calf zona glomerulosa cells were incubated with angiotensin II (A-II) and increasing concentrations of the ANP, ProANP 1-30 and 31-67 to determine if their reported natriuretic activity was mediated through suppression of aldosterone secretion. ANP as reported by many investigators produced a dose-dependent and potent inhibition of A-II-mediated aldosterone secretion. The Pro-ANP 1-30 and 31-67 did not affect A-II-stimulated aldosterone secretion at any of the doses tested. This study shows that the reported natriuretic effect of the fragments is not mediated by inhibition of aldosterone secretion.
前心钠素（Pro-ANP）是一种由126个氨基酸组成的肽，心钠素（ANP）（99-126个氨基酸）由其衍生而来。ANP具有强大的利尿、利钠和血管舒张作用。ANP还是醛固酮分泌的一种强大的直接和间接抑制剂。已证明，含有1-30和31-67肽段的心钠素原激素N端具有利尿、利钠和血管舒张特性。将分散的小牛肾小球带细胞与血管紧张素II（A-II）以及浓度不断增加的心钠素、ProANP 1-30和31-67一起孵育，以确定它们所报道的利钠活性是否通过抑制醛固酮分泌介导。正如许多研究人员所报道的，心钠素对A-II介导的醛固酮分泌产生剂量依赖性的强大抑制作用。在任何测试剂量下，Pro-ANP 1-30和31-67均不影响A-II刺激的醛固酮分泌。这项研究表明，所报道的这些片段的利钠作用并非由抑制醛固酮分泌介导。
