Rambotti M G, Ercolani P, Spreca A
Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Histochem J. 1993 May;25(5):384-91. doi: 10.1007/BF00159503.
We studied the cytochemical localization of particulate guanylate cyclase (GC) in rat adrenal gland after stimulation with porcine brain natriuretic peptide (pBNP) by electron microscopy. In the adrenal cortex, GC activity, as demonstrated by the presence of reaction product, was prevalently localized to the zona glomerulosa and zona fasciculata, while the zona reticularis showed little GC reaction product. In the adrenal medulla, GC reaction product was present only in adrenalin-containing cells. All GC positivity was associated with intracellular membranes. No GC reaction product was detected in specimens incubated in media devoid of pBNP. In parallel samples incubated in the presence of rat atrial natriuretic factor (rANF), the distribution of rANF-stimulated GC activity was similar to that of pBNP-stimulated GC activity.
我们通过电子显微镜研究了用猪脑钠肽（pBNP）刺激后大鼠肾上腺中颗粒型鸟苷酸环化酶（GC）的细胞化学定位。在肾上腺皮质中，如反应产物所示，GC活性主要定位于球状带和束状带，而网状带显示很少的GC反应产物。在肾上腺髓质中，GC反应产物仅存在于含肾上腺素的细胞中。所有GC阳性均与细胞内膜相关。在不含pBNP的培养基中孵育的标本中未检测到GC反应产物。在存在大鼠心房钠尿肽（rANF）的情况下孵育的平行样本中，rANF刺激的GC活性分布与pBNP刺激的GC活性分布相似。
