Hambor J E, Weber M C, Tykocinski M L, Kaplan D R
Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106.
Int Immunol. 1990;2(9):879-83. doi: 10.1093/intimm/2.9.879.
By antisense-mediated inhibition of CD8 expression in T cell clones and expression of CD8 in non-T cell lines, we have produced several sets of CD8+/CD8- paired cell lines. These cellular reagents have allowed us to assess the effect of CD8 surface expression on the immunogenic potential of stimulator cells. We found that the presence of CD8 on stimulator cells markedly decreased their capacity to stimulate proliferative responses or to induce the generation of cytotoxic activity. The CD8 alpha chain, in the absence of the beta chain, was sufficient to mediate this inhibitory effect. Our findings support the notion that CD8 functions as an immunoregulatory ligand and that auxiliary cell surface molecules on stimulator cells can have profound effects on the immunogenic capabilities of these cells.
通过反义介导抑制T细胞克隆中CD8的表达以及在非T细胞系中表达CD8，我们构建了几组CD8+/CD8-配对细胞系。这些细胞试剂使我们能够评估CD8表面表达对刺激细胞免疫原性潜力的影响。我们发现刺激细胞上CD8的存在显著降低了它们刺激增殖反应或诱导细胞毒活性产生的能力。在没有β链的情况下，CD8α链足以介导这种抑制作用。我们的研究结果支持这样的观点，即CD8作为一种免疫调节配体发挥作用，并且刺激细胞上的辅助细胞表面分子可对这些细胞的免疫原性能力产生深远影响。
