Kaplan D R, Hambor J E, Tykocinski M L
Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106.
Proc Natl Acad Sci U S A. 1989 Nov;86(21):8512-5. doi: 10.1073/pnas.86.21.8512.
The molecular details of immunoregulatory phenomena associated with CD8+ T lymphocytes have not been clearly elucidated. We tested the hypothesis that the cell surface glycoprotein CD8 is itself essential in mediating the inhibitory effects associated with CD8+ T cells. For this purpose we utilized a T-cell clonal pair, consisting of a human CD8+ T-cell clone and a specific CD8- phenocopy of this clone obtained via antisense RNA mutagenesis, to modulate allogeneic responses in vitro. Our findings indicate that the expression of the CD8 molecule by the inhibitory cells is essential for down-regulation of both allogeneic proliferation and generation of cytotoxicity in mixed lymphocyte cultures. These results define an immunomodulatory function for the CD8 molecule and provide insights into the molecular basis of immunosuppression.
与CD8 + T淋巴细胞相关的免疫调节现象的分子细节尚未得到明确阐明。我们检验了这样一个假说，即细胞表面糖蛋白CD8本身在介导与CD8 + T细胞相关的抑制作用中至关重要。为此，我们利用了一个T细胞克隆对，它由一个人CD8 + T细胞克隆和通过反义RNA诱变获得的该克隆的特异性CD8 - 表型模拟物组成，以在体外调节同种异体反应。我们的研究结果表明，抑制性细胞表达CD8分子对于下调混合淋巴细胞培养物中的同种异体增殖和细胞毒性的产生至关重要。这些结果定义了CD8分子的免疫调节功能，并为免疫抑制的分子基础提供了见解。
