Department of Radiology, Mount Auburn Hospital, 330 Mount Auburn Street, Cambridge, MA 02138, USA.
Mol Imaging. 2010 Oct;9(5):278-90.
The purpose of this study was to compare viable and nonviable bilabeled mesenchymal stem cells (MSCs) in arthritic joints with magnetic resonance imaging (MRI) and optical imaging (OI). MSCs were labeled with ferucarbotran and DiD. MRI and OI of bilabeled cells were compared with controls. Six rats with arthritis received intra-articular injections of bilabeled viable MSCs into the right knee and nonviable MSCs into the left knee. Animals underwent MRI and OI preinjection and at 4, 24, 48, and 72 hours postinjection. The results were analyzed with a mixed random effects model and Fisher probability. Bilabeled MSCs showed increased MRI and OI signals compared to unlabeled controls (p < .0001). After intra-articular injection, bilabeled MSCs caused significant T2 and T2* effect on MRI and fluorescence on OI up to 72 hours postinjection (p < .05). There was no significant difference between viable and nonviable MSC signal in the knee joints; however, some of the viable cells migrated to an adjacent inflamed ankle joint (p < .05). Immunohistochemistry confirmed viable MSCs in right knee and ankle joints and nonviable MSCs in the left knee. Viable and nonviable cells could not be differentiated with MRI or OI signal intensity but were differentiated based on their ability to migrate in vivo.
本研究旨在通过磁共振成像（MRI）和光学成像（OI）比较关节炎关节中活细胞和死细胞双标记间充质干细胞（MSCs）。MSCs 用 Ferucarbotran 和 DiD 进行标记。将双标记活细胞和死细胞的 MRI 和 OI 与对照进行比较。6 只关节炎大鼠的右膝关节内注射双标记活 MSCs，左膝关节内注射非活 MSCs。动物在注射前、注射后 4、24、48 和 72 小时进行 MRI 和 OI。结果采用混合随机效应模型和 Fisher 概率进行分析。与未标记对照相比，双标记 MSCs 显示出增强的 MRI 和 OI 信号（p<0.0001）。关节内注射后，双标记 MSCs 在注射后 72 小时内对 MRI 产生显著的 T2 和 T2*效应，对 OI 产生荧光效应（p<0.05）。膝关节中活细胞和死细胞的信号无显著差异；然而，一些活细胞迁移到相邻的发炎踝关节（p<0.05）。免疫组织化学证实右膝关节和踝关节中有活的 MSCs，左膝关节中有非活的 MSCs。活细胞和死细胞不能通过 MRI 或 OI 信号强度来区分，但可以根据其在体内迁移的能力来区分。
