Brizzio Aníbal A, Tedeschi Fabián A, Zalazar Fabián E
Servicio de Laboratorio, Agencia Santafesina de Seguridad Alimentaria (ASSAL), 3000 Santa Fe.
Rev Argent Microbiol. 2011 Jan-Mar;43(1):28-32. doi: 10.1590/S0325-75412011000100006.
On February 2008, a suspected foodborne outbreak was reported in Las Rosas (Santa Fe Province, Argentina). The formal procedures indicated that an undetermined number of individuals had experienced food poisoning following consumption of vegetable cannelloni bought at a local shop. The manufacturer establishment was audited. Samples from the suspected food, as well as environmental samples and swabs from food handlers were obtained and involved subjects were interviewed. Remnants of ingested food were also obtained. Routine microbiological procedures of the foodborne outbreak revealed the presence of coagulase positive S. aureus subspecies aureus in samples from ingested and raw food, and from manipulators. Indicator microorganisms did not show significant levels and no other foodborne pathogen was isolated. Presence of staphylococcal enterotoxin-producing genes was subsequently investigated, and a positive result for enterotoxin B was shown in S. aureus strains isolated from a food handler as well as from food linked to the outbreak Moreover, these isolates showed 100% similarity by SmaI-PFGE. Timely notification together with coordinated sanitary measures and the availability of appropriate laboratory tools allowed to interrupt the chain of disease transmission by identifying risk and protective factors.
2008年2月,阿根廷圣菲省拉斯罗萨斯报告了一起疑似食源性疾病暴发事件。正式程序表明,有数量不明的个人在食用了从当地一家商店购买的蔬菜意大利饺子后出现食物中毒症状。对生产厂家进行了审核。采集了疑似食品样本、环境样本以及食品处理人员的拭子样本,并对相关人员进行了访谈。还获取了摄入食物的残留物。食源性疾病暴发的常规微生物学检测程序显示,在摄入食物、生食以及食品处理人员的样本中存在凝固酶阳性金黄色葡萄球菌金黄色亚种。指示微生物未显示出显著水平,未分离出其他食源性病原体。随后对产生葡萄球菌肠毒素的基因进行了调查,从一名食品处理人员以及与此次暴发相关的食品中分离出的金黄色葡萄球菌菌株显示出肠毒素B呈阳性结果。此外,这些分离株通过SmaI - PFGE显示出100%的相似性。及时通报、协调一致的卫生措施以及合适的实验室工具的可用性,通过识别风险和保护因素,得以中断疾病传播链。