Nutrition Postgraduate Program, Health Sciences Center, Federal University of Rio Grande do Norte (UFRN), Natal, RN, Brazil.
Department of Pharmacy, Health Sciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
PLoS One. 2023 Feb 21;18(2):e0282111. doi: 10.1371/journal.pone.0282111. eCollection 2023.
Food contamination by Staphylococcus spp. enterotoxigenic strains is quite common and despite underreporting caused by the short duration of clinical symptoms and lack of medical care, staphylococcal food poisoning is one of the most common Foodborne Diseases (FBD) in the world. This study describes a systematic review protocol with meta-analysis on the prevalence and types of staphylococcal enterotoxins in food, and the profile of contaminated foods.
The research will be conducted through the selection of studies reporting the analysis of staphylococcal enterotoxins in food contaminated by Staphylococcus spp. Searches will happen on the following databases: Medline (OVID), GALE, Science Direct, CAB Direct (CABI), Google Scholar, in addition to manual search in the list of references of articles, directory of theses and dissertations, and countries' health agencies. Reports will be imported into the application Rayyan. Two researchers will independently select studies and extract data, and a third reviewer will solve conflicting decisions. The primary outcome will be the identification of staphylococcal enterotoxins in food, and the secondary outcomes will include staphylococcal enterotoxin types and foods involved. To assess the risk of bias in the studies, the tool developed by the Joanna Briggs Institute (JBI) will be used. For data synthesis, a meta-analysis will be performed. However, in case that is not possible, a narrative synthesis of the most relevant results will be carried out.
This protocol will serve as the basis for a systematic review that aims to relate the results of existing studies on the staphylococcal enterotoxin prevalence and types in food, and the profile of the contaminated foods. The results will broaden the perception of food safety risks, highlight existing literature gaps, contribute to the study of the epidemiological profile and may guide the allocation of health resources for the development of preventive measures related.
PROSPERO registration number: CRD42021258223.
食品中金黄色葡萄球菌（Staphylococcus spp.）肠毒素产生菌的污染相当普遍，尽管由于临床症状持续时间短且缺乏医疗护理而导致报告不足，但葡萄球菌食物中毒仍是世界上最常见的食源性疾病之一。本研究描述了一项系统评价方案，包括对食品中金黄色葡萄球菌肠毒素的流行率和类型以及污染食品的特征进行荟萃分析。
研究将通过选择报告金黄色葡萄球菌污染食品中肠毒素分析的研究来进行。将在以下数据库中进行搜索：Medline（OVID）、GALE、Science Direct、CAB Direct（CABI）、Google Scholar，此外还将在文章参考文献列表、论文目录和国家卫生机构中进行手动搜索。报告将被导入到应用程序 Rayyan 中。两名研究人员将独立选择研究并提取数据，第三名评审员将解决有争议的决策。主要结果将是鉴定食品中的肠毒素，次要结果将包括肠毒素类型和涉及的食品。为了评估研究中的偏倚风险，将使用 Joanna Briggs 研究所（JBI）开发的工具。对于数据综合，将进行荟萃分析。然而，如果不可能，则将对最相关的结果进行叙述性综合。
本方案将作为系统评价的基础，旨在关联现有研究中关于食品中肠毒素的流行率和类型以及污染食品特征的结果。结果将扩大对食品安全风险的认识，突出现有文献差距，有助于研究流行病学特征，并可能为开发相关预防措施指导卫生资源的分配。
PROSPERO 注册号：CRD42021258223。
