Department of Biomedical Science and Biotechnology, University of Brescia, Brescia, Italy.
J Clin Immunol. 2011 Aug;31(4):540-9. doi: 10.1007/s10875-011-9526-6. Epub 2011 Apr 14.
The study aims to obtain more information about the immune deficit of common variable immunodeficiency (CVID) patients.
A new real-time PCR assay was used to quantify T and B lymphocyte mobilization from the production and maturation sites through the detection of T cell receptor excision circles (TRECs) and kappa-deleting recombination circles (KRECs) and to allow the estimation of the average number of B cell divisions. T and B lymphocyte subsets were analyzed by flow cytometry.
The number of TREC(+) lymphocytes, which depends on age and gender, was significantly reduced in CVID patients. Similarly, KREC concentration was lower than in controls. Classification of patients according to the percentage of memory switched B cells showed that patients belonging to MB2 group and therefore with conserved B cell maturation have the lowest new B cell output but increased average peripheral divisions, leading to the highest B cell number.
TREC and KREC quantification can be helpful for a more complete and informative understanding of a heterogeneous disease such as CVID.
本研究旨在更深入地了解普通可变免疫缺陷（CVID）患者的免疫缺陷。
采用新型实时 PCR 检测方法，通过检测 T 细胞受体切除环（TRECs）和 κ 缺失重组环（KRECs），从生成和成熟部位定量 T 和 B 淋巴细胞的动员情况，从而估计 B 细胞的平均分裂次数。采用流式细胞术分析 T 和 B 淋巴细胞亚群。
依赖于年龄和性别的 TRECs(+)淋巴细胞数量在 CVID 患者中显著减少。同样，KREC 浓度也低于对照组。根据记忆性 B 细胞转换百分比对患者进行分类，结果表明属于 MB2 组的患者（即 B 细胞成熟得到保留）的新 B 细胞输出量最低，但外周平均分裂增加，导致 B 细胞数量最高。
TREC 和 KREC 的定量检测有助于更全面、更深入地了解 CVID 等异质性疾病。
