Østergaard Michael E, Wamberg Michael C, Pedersen Erik B
Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Odense M, Denmark.
Nucleosides Nucleotides Nucleic Acids. 2011 Mar;30(3):210-26. doi: 10.1080/15257770.2011.567202.
We have synthesized a carbon linker analogue of INA (oligonucleotides containing insertions of 1-O-(1-pyrenylmethyl)glycerol). Thermal stability studies showed an increase in melting temperature in favor of the carbon linker analogue. We also synthesized a carbon linker analogue with two pyrenes geminally attached. Fluorescence studies of this intercalating nucleic acid with the pyrene moieties inserted as a bulge showed formation of an excimer band. When a mismatch was introduced at the site of the intercalator, an excimer band was formed for the destabilized duplexes whereas an exciplex band was observed when the stability of the duplex was retained.
我们合成了异核苷酸钠（INA）的碳连接类似物（含有1-O-(1-芘甲基)甘油插入片段的寡核苷酸）。热稳定性研究表明，其解链温度升高，有利于碳连接类似物。我们还合成了一种带有两个相连芘基团的碳连接类似物。对这种插入芘部分形成凸起的嵌入核酸进行荧光研究，结果显示形成了准分子带。当在嵌入剂位点引入错配时，不稳定双链体形成准分子带，而当双链体稳定性得以保留时，则观察到激基复合物带。
