Office of the Texas State Chemist, Texas AgriLife Research, Texas A&M University, College Station, TX 77843, USA.
Rapid Commun Mass Spectrom. 2011 May 15;25(9):1222-30. doi: 10.1002/rcm.4979.
The objective of the present study is to develop a simple, fast method for detection of aflatoxins in animal feeds. Simultaneous quantitation of four aflatoxins (AFB(1), AFB(2), AFG(1) and AFG(2)) in animal feeds was achieved in a single liquid chromatography/tandem mass spectrometry (LC/MS/MS) run. The solid-phase extraction cleanup step is eliminated with the stable isotope dilution method. Matrix effects were observed and overcome by isotope dilution. The method was tested in a variety of animal feed matrices and proved to be accurate and reliable. Method ruggedness tests resulted in recoveries of 78% to 122% with an intra-day assay precision of 2% to 15% and an inter-day assay precision of 3% to 17%. These results indicate that this method is suitable for quantitation of aflatoxins in animal feeds.
本研究的目的是开发一种简单、快速的方法来检测动物饲料中的黄曲霉毒素。在单次液相色谱/串联质谱（LC/MS/MS）运行中，同时定量了四种黄曲霉毒素（AFB(1)、AFB(2)、AFG(1)和 AFG(2)）。采用稳定同位素稀释法消除固相萃取净化步骤。通过同位素稀释克服了基质效应。该方法在多种动物饲料基质中进行了测试，结果证明准确可靠。方法稳健性测试的回收率为 78%至 122%，日内测定精密度为 2%至 15%，日间测定精密度为 3%至 17%。这些结果表明，该方法适用于动物饲料中黄曲霉毒素的定量。
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