Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China.
Toxins (Basel). 2021 Nov 26;13(12):844. doi: 10.3390/toxins13120844.
This study was performed to assess the effects of dietary aflatoxin B (AFB) on the growth, antioxidant and immune response, digestive enzyme activities, and intestinal morphology of during a 56-day feeding trial. Four diets were formulated including 0, 0.1, 0.5, and 1.0 mg/kg of AFB. Each diet was randomly assigned to 3 fish tanks with 40 fish per tank. Results indicated that the fish's final body weight, weight gain rate, specific growth rate, feed intake, condition factor, viscerosomatic index, hepatosomatic index, and intestinesomatic index decreased ( < 0.01) as dietary AFB increased. AFB levels in diets increased ( < 0.05) serum total antioxidant capacity (TAOC), superoxide (SOD), catalase, malondialdehyde (MDA), alkaline phosphatase (AKP), and lysozyme (LZM), and increased ( < 0.05) the TAOC, SOD, MDA, AKP, LZM, and immunoglobulin M in the livers of the fish. Dietary AFB decreased ( < 0.05) intestinal trypsin activity and induced intestinal injury. In summary, dietary AFB up to 1.0 mg/kg was toxic to as judged by reduced growth, enhanced antioxidant and immune response, decreased intestinal trypsin activity, and impaired intestinal morphology.
本研究旨在评估饲料黄曲霉毒素 B (AFB) 对鲤鱼生长、抗氧化和免疫反应、消化酶活性以及肠道形态的影响。在为期 56 天的饲养试验中，设计了四种饲料，分别含有 0、0.1、0.5 和 1.0 mg/kg 的 AFB。每种饲料随机分配到 3 个鱼缸中，每个鱼缸有 40 条鱼。结果表明，随着饲料 AFB 的增加，鱼的最终体重、增重率、特定生长率、饲料摄入量、体况系数、内脏体比、肝体比和肠体比均降低（<0.01）。饲料中 AFB 水平升高（<0.05）血清总抗氧化能力（TAOC）、超氧化物歧化酶（SOD）、过氧化氢酶、丙二醛（MDA）、碱性磷酸酶（AKP）和溶菌酶（LZM），并升高（<0.05）鱼肝脏中的 TAOC、SOD、MDA、AKP、LZM 和免疫球蛋白 M。饲料 AFB 降低（<0.05）肠道胰蛋白酶活性并诱导肠道损伤。总之，日粮 AFB 高达 1.0 mg/kg 对鲤鱼是有毒的，表现为生长减缓、抗氧化和免疫反应增强、肠道胰蛋白酶活性降低以及肠道形态受损。
