State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
Macromol Rapid Commun. 2011 Jun 16;32(12):899-904. doi: 10.1002/marc.201100108. Epub 2011 Apr 12.
In this communication, the application of coordination polymer nanobelts (CPNs) assembled from H(2)PtCl(6) and 3,3',5,5'-tetramethylbenzidine (TMB) are explored as an effective fluorescent sensing platform for nucleic acid detection for the first time. The suggested method has a high selectivity down to single-base mismatch. DNA detection is accomplished by the following two steps: (1) CPN binds fluorecent dye-labeled single-stranded DNA (ssDNA) probe via both electrostatic attraction and π-π stacking interactions between unpaired DNA bases and CPN. As a result, the fluorescent dye is brought into close proximity to CPN and substantial fluorescence quenching occurs due to photoinduced electron transfer from the nitrogen atom in CPN to the excited fluorophore. (2) The hybridization of adsorbed ssDNA probe with its target generates a double stranded DNA (dsDNA). The duplex cannot be adsorbed by CPN due to its rigid conformation and the absence of unpaired DNA bases, leading to an obvious fluorescence enhancement.
在本通讯中，首次探索了由 H(2)PtCl(6) 和 3,3',5,5'-四甲基联苯胺 (TMB) 组装的配位聚合物纳带 (CPN) 在核酸检测中的应用，作为一种有效的荧光传感平台。所提出的方法具有很高的选择性，甚至可以检测到单个碱基错配。DNA 检测分两步进行：(1)CPN 通过静电吸引和未配对 DNA 碱基与 CPN 之间的 π-π 堆积相互作用与荧光染料标记的单链 DNA (ssDNA) 探针结合。结果，荧光染料被带入 CPN 的近邻，由于从 CPN 中的氮原子到激发荧光团的光致电子转移，发生了大量的荧光猝灭。(2)吸附的 ssDNA 探针与靶标杂交生成双链 DNA (dsDNA)。由于其刚性构象和缺少未配对的 DNA 碱基，双链 DNA 不能被 CPN 吸附，导致明显的荧光增强。
