Chang Guohui, Hu Jianming, Lu Wenbo, Qin Xiaoyun, Asiri Abdullah M, Al-Youbi Abdulrahman O, Sun Xuping
Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical Industry, China West Normal University, Nanchong 637002, Sichuan, China.
J Nanosci Nanotechnol. 2012 May;12(5):3775-80. doi: 10.1166/jnn.2012.5848.
In this communication, we demonstrate the proof of concept that carbon nanocapsules (CNCs) can be used as an effective fluorescent sensing platform for nucleic acid detection with selectivity down to single-base mismatch. The detection is accomplished by two steps: (1) CNC adsorbs and quenches the fluorescence of the dye-labeled single-stranded DNA (ssDNA) probe; (2) in the presence of the target, a hybridization event occurs, which produces a double-stranded DNA (dsDNA) that detaches from the CNC surface, leading to recovery of the dye fluorescence.
在本通讯中，我们证明了碳纳米胶囊（CNCs）可作为一种有效的荧光传感平台用于核酸检测，其选择性可达单碱基错配水平的概念验证。检测通过两个步骤完成：（1）CNC吸附并淬灭染料标记的单链DNA（ssDNA）探针的荧光；（2）在靶标存在的情况下，发生杂交事件，产生从CNC表面脱离的双链DNA（dsDNA），从而导致染料荧光恢复。
