Institute for Bioprocessing and Analytical Measurement Techniques, Rosenhof, Germany.
Biomacromolecules. 2011 Jun 13;12(6):1987-97. doi: 10.1021/bm200258q. Epub 2011 May 3.
To promote osteoblast adhesion and proliferation on (bio)material surfaces, biomimetic coatings resembling the natural extracellular matrix (ECM) are desirable. The glycosamino glycans (GAGs) chondroitin sulfate (CS) and heparin (HEP) are promising candidates for a biomimetic coating since they are two of the most prevalent noncollagenous biomolecules constituting the ECM. Coatings containing CS and HEP were prepared employing the "layer by layer" technique yielding polyelectrolyte multilayers (PEMs). Physicochemical and mechanical characterization of the coatings were performed by means of streaming potential measurements and colloidal force spectroscopy. The capability of the coatings to support cell adhesion, spreading, proliferation, and maintenance of an osteoblastic phenotype was assessed with SaOS osteosarcoma cells. We demonstrate that PEMs constructed from CS as the polyanion display a low Young's modulus correlated with poorly supported cell adhesion and proliferation. When the CS was adsorbed onto a stiffer polypeptide PEM basis, the Young's modulus increased, and the cell response was significantly improved. For HEP coatings an intermediate Young's modulus and moderate cell adhesion and spreading were observed. No significant changes in stiffness or cell response were detected when HEP was adsorbed onto the polypeptide film.
为了促进（生物）材料表面成骨细胞的黏附和增殖，理想的是具有类似天然细胞外基质（ECM）的仿生涂层。糖胺聚糖（GAG）硫酸软骨素（CS）和肝素（HEP）是仿生涂层的有前途的候选物，因为它们是构成 ECM 的两种最普遍的非胶原蛋白生物分子。使用“层层”技术制备了含有 CS 和 HEP 的涂层，得到了聚电解质多层（PEM）。通过流动电势测量和胶体力谱法对涂层的物理化学和机械特性进行了表征。通过 SaOS 骨肉瘤细胞评估了涂层支持细胞黏附、铺展、增殖和维持成骨表型的能力。我们证明，作为聚阴离子的 CS 构建的 PEM 具有低杨氏模量，与支持不良的细胞黏附和增殖相关。当 CS 吸附到更硬的多肽 PEM 基底上时，杨氏模量增加，细胞反应得到显著改善。对于 HEP 涂层，观察到中等杨氏模量和适度的细胞黏附和铺展。当 HEP 吸附到多肽膜上时，没有检测到刚度或细胞反应的显著变化。
