Department of Biomaterials, Institute for Bioprocessing and Analytical Measurement Techniques (IBA), Rosenhof, 37308 Heilbad Heiligenstadt, Germany.
Biointerphases. 2011 Jun;6(2):54-62. doi: 10.1116/1.3589176.
Coatings of biomaterials or implants that facilitate biomineralization possess a great potential for applications focused to the replacement, augmentation, and regeneration of bone tissue. Biomimetic approaches utilize biomolecules for either templating or supporting the crystallization process. One of these promising biomolecules is phosvitin (PV), an egg yolk protein known to transport and store inorganic phosphates and calcium ions. The incorporation of PV into polyelectrolyte multilayers is favorable due to PVs high degree of phosphorylation and thus a high acidity. Utilizing the reflectometric interference spectroscopy, the adsorption kinetics of this novel polyelectrolyte system composed of poly-L-lysine and the heavily phosphorylated phosvitin were monitored. The results demonstrate an unexpected nonregular growth regime called overshoot. Effective measures of shifting this irregular polyelectrolyte adsorption process back to a regular multilayer growth regime are reported in this paper.
具有促进生物矿化作用的生物材料或植入物涂层在专注于替代、增强和再生骨组织的应用中具有巨大的潜力。仿生方法利用生物分子进行模板或支持结晶过程。其中一种有前途的生物分子是卵黄蛋白（PV），这是一种已知能够转运和储存无机磷酸盐和钙离子的蛋黄蛋白。由于 PV 高度磷酸化，因此具有很高的酸度，因此将 PV 掺入聚电解质多层中是有利的。利用反射干涉光谱法，监测了由聚-L-赖氨酸和高度磷酸化的卵黄蛋白组成的新型聚电解质体系的吸附动力学。结果表明存在一种称为过冲的非规则生长状态。本文报道了有效措施，可将这种不规则的聚电解质吸附过程转移回规则的多层生长状态。
