Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, 100048 Beijing, China.
Genome. 2011 Apr;54(4):273-84. doi: 10.1139/g10-119.
Phylogenetic relationships between the C, U, N, and M genomes of Aegilops species and the genomes of common wheat and other related species were investigated by using three types of low-molecular-weight glutenin subunit (LMW-GS) genes at Glu-3 loci. A total of 20 LMW-GS genes from Aegilops and Triticum species were isolated, including 11 LMW-m type and 9 LMW-i type genes. Particularly, four LMW-m type and three LMW-i type subunits encoded by the genes on the C, N, and U genomes possessed an extra cysteine residue at conserved positions, which could provide useful information for understanding phylogenetic relationships among Aegilops and Triticum genomes. Phylogenetic trees constructed by using either LMW-i or the combination of LMW-m and LMW-s, as well as analysis of all the three types of LMW-GS genes together, demonstrated that the C and U genomes were closely related to the A genome, whereas the N and M genomes were closely related to the D genome. Our results support previous findings that the A genome was derived from Triticum uratu, the B genome was from Aegilops speltoides, and the D genome was from Aegilops tauschii. In addition, phylogenetic relationships among different genomes analysed in this study support the concept that Aegilops is not monophyletic.
利用 Glu-3 位点的三种低分子量谷蛋白亚基 (LMW-GS) 基因，研究了普通小麦及其它相关物种的 C、U、N 和 M 基因组与小麦族物种的系统发育关系。从小麦族和黑麦族物种中分离出了 20 种 LMW-GS 基因，包括 11 种 LMW-m 型和 9 种 LMW-i 型基因。特别是，在 C、N 和 U 基因组基因编码的四个 LMW-m 型和三个 LMW-i 型亚基中，在保守位置上都含有一个额外的半胱氨酸残基，这为理解小麦族和黑麦族基因组之间的系统发育关系提供了有用的信息。利用 LMW-i 或 LMW-m 和 LMW-s 的组合，以及同时分析三种类型的 LMW-GS 基因构建的系统发育树表明，C 和 U 基因组与 A 基因组密切相关，而 N 和 M 基因组与 D 基因组密切相关。我们的研究结果支持了先前的发现，即 A 基因组来自普通小麦，B 基因组来自节节麦，D 基因组来自粗山羊草。此外，本研究分析的不同基因组之间的系统发育关系支持了小麦族不是单系的概念。
