Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brazil.
Can J Microbiol. 2011 Apr;57(4):333-8. doi: 10.1139/w11-011.
Trichophyton rubrum is the most common etiological agent of human dermatophytosis. Despite the incidence and medical importance of this dermatophyte, little is known about the mechanisms of host invasion and pathogenicity. Host invasion depends on the adaptive cellular responses of the pathogen that allow it to penetrate the skin layers, which are mainly composed of proteins and lipids. In this study, we used suppression subtractive hybridization to identify transcripts overexpressed in T. rubrum cultured in lipid as carbon source. Among the subtractive cDNA clones isolated, 85 clones were positively screened by cDNA array dot blotting and were sequenced. The putative proteins encoded by the isolated transcripts showed similarities to fungal proteins involved in metabolism, signaling, defense, and virulence, such as the MDR/ABC transporter, glucan 1,3-β-glucosidase, chitin synthase B, copper-sulfate-regulated protein, and serine/threonine phosphatase (calcineurin A). These results provide the first molecular insight into the genes differentially expressed during the adaptation of T. rubrum to a lipidic carbon source.
红色毛癣菌是引起人类皮肤癣菌病的最常见病原体。尽管这种皮肤癣菌的发病率和医学重要性很高，但人们对其宿主入侵和致病性的机制知之甚少。宿主入侵依赖于病原体的适应性细胞反应，使它能够穿透主要由蛋白质和脂质组成的皮肤层。在这项研究中，我们使用抑制性消减杂交技术来鉴定在以脂质作为碳源培养的红色毛癣菌中过表达的转录本。在分离的消减 cDNA 克隆中，85 个克隆通过 cDNA 阵列斑点印迹被阳性筛选，并进行了测序。由分离的转录本编码的假定蛋白与参与代谢、信号转导、防御和毒力的真菌蛋白具有相似性，如 MDR/ABC 转运蛋白、葡聚糖 1,3-β-葡糖苷酶、几丁质合成酶 B、硫酸铜调节蛋白和丝氨酸/苏氨酸磷酸酶（钙调神经磷酸酶 A）。这些结果首次提供了对红色毛癣菌适应脂质碳源时差异表达基因的分子见解。
