Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
BMC Microbiol. 2010 Feb 8;10:39. doi: 10.1186/1471-2180-10-39.
Cutaneous mycoses are common human infections among healthy and immunocompromised hosts, and the anthropophilic fungus Trichophyton rubrum is the most prevalent microorganism isolated from such clinical cases worldwide. The aim of this study was to determine the transcriptional profile of T. rubrum exposed to various stimuli in order to obtain insights into the responses of this pathogen to different environmental challenges. Therefore, we generated an expressed sequence tag (EST) collection by constructing one cDNA library and nine suppression subtractive hybridization libraries.
The 1388 unigenes identified in this study were functionally classified based on the Munich Information Center for Protein Sequences (MIPS) categories. The identified proteins were involved in transcriptional regulation, cellular defense and stress, protein degradation, signaling, transport, and secretion, among other functions. Analysis of these unigenes revealed 575 T. rubrum sequences that had not been previously deposited in public databases.
In this study, we identified novel T. rubrum genes that will be useful for ORF prediction in genome sequencing and facilitating functional genome analysis. Annotation of these expressed genes revealed metabolic adaptations of T. rubrum to carbon sources, ambient pH shifts, and various antifungal drugs used in medical practice. Furthermore, challenging T. rubrum with cytotoxic drugs and ambient pH shifts extended our understanding of the molecular events possibly involved in the infectious process and resistance to antifungal drugs.
皮肤真菌病是健康和免疫功能低下宿主中常见的人类感染，亲人性真菌红色毛癣菌是全球此类临床病例中最常分离到的微生物。本研究旨在确定红色毛癣菌暴露于各种刺激物时的转录谱，以深入了解该病原体对不同环境挑战的反应。因此，我们构建了一个 cDNA 文库和九个抑制性消减杂交文库，生成了一个表达序列标签 (EST) 集。
本研究中鉴定的 1388 个基因根据慕尼黑信息中心蛋白质序列 (MIPS) 类别进行了功能分类。鉴定的蛋白质参与转录调控、细胞防御和应激、蛋白质降解、信号转导、运输和分泌等功能。对这些基因的分析揭示了 575 个红色毛癣菌序列以前未在公共数据库中记录。
在本研究中，我们鉴定了新的红色毛癣菌基因，这将有助于在基因组测序中进行 ORF 预测，并促进功能基因组分析。这些表达基因的注释揭示了红色毛癣菌对碳源、环境 pH 值变化和医学实践中使用的各种抗真菌药物的代谢适应。此外，用细胞毒性药物和环境 pH 值变化挑战红色毛癣菌，扩展了我们对感染过程和抗真菌药物耐药性中可能涉及的分子事件的理解。
