Faculty of Medicine and Dentistry, University of Murcia, Murcia, SpainDepartment of Prosthodontics, Faculty of Medicine, Stony Brook University, Stony Brook, NY.
Clin Oral Implants Res. 2012 Jun;23(6):667-675. doi: 10.1111/j.1600-0501.2011.02193.x. Epub 2011 Apr 15.
To carry out a histomorphometric analysis of a new highly porous (95%) biphasic calcium phosphate (hydroxyapatite 60%/B-tricalcium phosphate 40%), used to fill critical size defects in rabbit tibiae, supplementing histomorphometric findings with radiographic thermal imaging, EDX analysis and Ca/P ratio mapping at different time stages.
Two critical size defects of 6 mm diameter were created in both tibiae of 21 New Zealand rabbits, test group (Ossceram) and control group. Histomorphometric, radiographic thermal imaging, EDX and element mapping analysis were performed at 15, 30 and 60 days after graft insertion.
Histomorphometric analysis at 30 days showed more new bone formation in defects filled with Ossceram 4.41 ± 0.23 mm than the test group 1.94 ± 0. 28 mm (P<0.05). Element analysis revealed higher percentages of Ca (42.33 ± 2.8%) and P (1.3 ± 0.8%) in the test group than in the control group (P<0.05). Element mapping showed that Ca and P were concentrated in medullar and cortical zones in the test group but were concentrated only in cortical zones in the control group. Test group histomorphometry at 60 days showed complete closure of the cortical defect 5.37 ± 0.32 mm more than the control group 2.3 ± 0.54 mm. There was no cortical defect closure or medullar bone formation in the control group (P<0.05). Element analysis revealed higher percentages of Ca (32.26 ± 21.7%) and P (1.5 ± 0.3%) in the test group than in the control group (P<0.05).
Defects of a critical size in a rabbit tibia model can be sealed using a highly porous biphasic calcium phosphate; this supports new bone formation, creates a bridge between borders and facilitates bone ingrowth. Furthermore, this study observed partial dissolution of the mineral phase of the graft material and its incorporation into the surrounding bone. Radiographic thermal imaging may be used to supplement histological and chemical analyses.
对一种新型高多孔（95%）双相磷酸钙（羟基磷灰石 60%/β-磷酸三钙 40%）进行组织形态计量学分析，该材料用于填充兔胫骨的临界尺寸缺陷，通过 X 射线热成像、EDX 分析和 Ca/P 比映射补充组织形态计量学发现，以不同的时间阶段。
在 21 只新西兰兔的双侧胫骨中分别制作 6 毫米直径的两个临界尺寸缺陷，实验组（Ossceram）和对照组。在移植后 15、30 和 60 天进行组织形态计量学、X 射线热成像、EDX 和元素映射分析。
30 天的组织形态计量学分析显示，用 Ossceram 填充的缺陷中形成的新骨较多，为 4.41 ± 0.23 毫米，而对照组为 1.94 ± 0.28 毫米（P<0.05）。元素分析显示，实验组的 Ca（42.33 ± 2.8%）和 P（1.3 ± 0.8%）百分比高于对照组（P<0.05）。元素映射显示，实验组的 Ca 和 P 集中在骨髓和皮质区，但对照组仅集中在皮质区。实验组 60 天的组织形态计量学显示，皮质缺损完全闭合，为 5.37 ± 0.32 毫米，对照组为 2.3 ± 0.54 毫米。对照组无皮质缺损闭合或骨髓骨形成（P<0.05）。元素分析显示，实验组的 Ca（32.26 ± 21.7%）和 P（1.5 ± 0.3%）百分比高于对照组（P<0.05）。
兔胫骨模型的临界尺寸缺陷可用高多孔双相磷酸钙封闭；这支持新骨形成，在边界之间形成桥梁，并促进骨向内生长。此外，本研究观察到移植物材料的矿物质相部分溶解并融入周围骨骼。X 射线热成像可用于补充组织学和化学分析。
