Calvo-Guirado José Luis, Aguilar-Salvatierra Antonio, Ramírez-Fernández Maria P, Maté Sánchez de Val José E, Delgado-Ruiz Rafael Arcesio, Gómez-Moreno Gerardo
International Dentistry Research Cathedra, Universidad Católica San Antonio de Murcia (UCAM), Murcia, Spain.
Faculty of Dentistry, University of Granada, Granada, Spain.
Clin Oral Implants Res. 2016 Aug;27(8):1039-46. doi: 10.1111/clr.12681. Epub 2015 Oct 5.
This study aimed to carry out the evaluation of bone response of new bone formation to two different xenografts (bovine and porcine) biomaterials inserted in rabbit tibiae.
The study used a total of 20 male New Zealand albino rabbits. They received a total of 40 grafts in the proximal metaphyseal areas of both tibiae. Two biomaterials were evaluated: 20 porcine xenografts, as a bone granulate (OsteoBiol(®) MP3(®) ; Tecnoss srl, Giaveno, Italy), were placed in the proximal metaphyseal area of the right tibia, 20 anorganic bovine bone mineral grafting (4BONE(™) XBM, MIS Implants Inc., BARLEV, Israel) were placed in the left tibia. Following graft insertion, the animals were sacrificed in two groups of 10 animals, after 1 and 4 months, respectively. For each group, biomaterials were analyzed: newly formed bone, residual graft materials and the connective tissue. Histomorphometric, EDX analysis and element mapping were performed at 1 and 4 months after graft insertion.
At 4 months after treatment, the bone defects displayed radiological images that showed complete repair of osseous defects. Histomorphometric evaluation showed that for the porcine xenograft, the study averages for newly formed bone represented 84.23 ± 2.9%, while bovine matrix was 79.34 ± 2.1%. For residual graft material, the porcine biomaterial had 11.23 ± 1.7% and the bovine graft 31.56 ± 2.3%. Finally, the connective tissue for MP3 was 10.33 ± 1.8%, while for the 4BONE(™) XBM we obtained 14.34 ± 2.9%. Element analysis revealed higher percentages of Ca (54 ± 9%) and P (35 ± 6%) in the group B than group A and control group (P < 0.05).
Defects of a critical size in a rabbit tibia model can be sealed using a bovine porous biphasic calcium phosphate and MP3 material; this supports new bone formation, creates a bridge between borders, and facilitates bone ingrowth in both biomaterials. Furthermore, this study observed partial dissolution of the mineral phase of four bone graft and complete resorption of porcine MP3 biomaterial and its incorporation into the surrounding bone. Depending on clinical needs, each biomaterial could be useful in daily clinical practice.
本研究旨在评估两种不同异种移植物（牛和猪）生物材料植入兔胫骨后新骨形成的骨反应。
本研究共使用20只雄性新西兰白兔。它们在双侧胫骨近端干骺端区域共接受40处移植。评估两种生物材料：20处猪异种移植物，作为骨颗粒（OsteoBiol® MP3®；Tecnoss srl，Giaveno，意大利），置于右胫骨近端干骺端区域，20处无机牛骨矿物质移植（4BONE™ XBM，MIS Implants Inc.，BARLEV，以色列）置于左胫骨。移植后，分别在1个月和4个月后将动物分成两组，每组10只处死。对每组生物材料进行分析：新形成的骨、残留移植材料和结缔组织。在移植后1个月和4个月进行组织形态计量学、能量色散X射线分析（EDX）和元素映射。
治疗4个月后，骨缺损的放射影像显示骨缺损完全修复。组织形态计量学评估显示，对于猪异种移植物，新形成骨的研究平均值为84.23±2.9%，而牛基质为79.34±2.1%。对于残留移植材料，猪生物材料为11.23±1.
