Faculty of Agriculture and Life Sciences, P.O. Box 84 Lincoln University, Lincoln 7647, Canterbury, New Zealand.
Department of Chemistry, University of Canterbury, Private Bag 4800, Christchurch, New Zealand.
Biosens Bioelectron. 2011 May 15;26(9):3742-7. doi: 10.1016/j.bios.2011.02.011. Epub 2011 Feb 16.
Yeast microbial fuel cells have received little attention to date. Yeast should be ideal MFC catalyst because they are robust, easily handled, mostly non-pathogenic organisms with high catabolic rates and in some cases a broad substrate spectrum. Here we show that the non-conventional yeast Arxula adeninvorans transfers electrons to an electrode through the secretion of a reduced molecule that is not detectable when washed cells are first resuspended but which accumulates rapidly in the extracellular environment. It is a single molecule that accumulates to a significant concentration. The occurrence of mediatorless electron transfer was first established in a conventional microbial fuel cell and that phenomenon was further investigated by a number of techniques. Cyclic voltammetry (CV) on a yeast pellet shows a single peak at 450 mV, a scan rate study showed that the peak was due to a solution species. CVs of the supernatant confirmed a solution species. It appears that, given its other attributes, A. adeninivorans is a good candidate for further investigation as a MFC catalyst.
酵母微生物燃料电池目前受到的关注较少。酵母应该是理想的 MFC 催化剂，因为它们具有较强的生命力、易于操作、大多数是非致病性的生物体，具有较高的代谢率，在某些情况下还具有广泛的底物谱。在这里，我们发现非传统酵母 Arxula adeninvorans 通过分泌一种还原分子将电子传递到电极，当最初重新悬浮洗过的细胞时，这种还原分子是不可检测的，但它会在细胞外环境中迅速积累。这是一种会积累到显著浓度的单一分子。电子转移的无介体现象首先在传统微生物燃料电池中得到证实，并通过多种技术进一步研究了这一现象。酵母颗粒的循环伏安法 (CV) 在 450 mV 处显示出单个峰，扫描速率研究表明该峰是由于溶液中的一种物质。上清液的 CV 证实了一种溶液物质。鉴于其其他属性，A. adeninvorans 似乎是作为 MFC 催化剂进一步研究的良好候选物。
