刚地弓形虫强毒株和无毒株速殖子中核苷三磷酸水解酶的显著活性。

Remarkable activities of nucleoside triphosphate hydrolase in the tachyzoites of both virulent and avirulent strains of Toxoplasma gondii.

作者信息

Asai T, Suzuki Y

机构信息

Department of Microbiology, Tokyo Medical College, Japan.

出版信息

FEMS Microbiol Lett. 1990 Oct;60(1-2):89-92. doi: 10.1016/0378-1097(90)90350-y.

DOI:10.1016/0378-1097(90)90350-y

PMID:2149344

Abstract

Dithiothreitol-dependent MgATP2- and Mg-ADP- hydrolytic activities owing to nucleoside triphosphate hydrolase were examined kinetically in tachyzoite cells of five strains of Toxoplasma gondii with various virulence. The tachyzoites of all the strains were revealed to have a high ATP and ADP hydrolytic potency. The value of Vmax and Km obtained from each strain indicated that there were two classified types of T. gondii about ATP and ADP hydrolysis. The most virulent strain (RH) and avirulent strain (Fukaya) were classified in a type with higher ATPase than ADPase activities. Two virulent strains (C56, Beverley) and an avirulent strain (ME49) were classified in the other type with the same activities of ATPase and ADPase.

摘要

在五种不同毒力的刚地弓形虫速殖子细胞中，对核苷三磷酸水解酶所具有的依赖二硫苏糖醇的MgATP2 - 和Mg - ADP水解活性进行了动力学研究。结果显示，所有菌株的速殖子都具有较高的ATP和ADP水解能力。从各菌株获得的Vmax和Km值表明，刚地弓形虫在ATP和ADP水解方面存在两种分类类型。最强毒株（RH）和无毒株（深谷株）归为一类，其ATP酶活性高于ADP酶活性。两种毒株（C56、贝弗利株）和一种无毒株（ME49）归为另一类，其ATP酶和ADP酶活性相同。